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Membrane and lipid metabolism plays an important role in desiccation resistance in the yeast Saccharomyces cerevisiae
- Source :
- BMC Microbiology, BMC Microbiology, Vol 20, Iss 1, Pp 1-13 (2020)
- Publication Year :
- 2020
- Publisher :
- BioMed Central, 2020.
-
Abstract
- Background Anhydrobiotes, such as the yeast Saccharomyces cerevisiae, are capable of surviving almost total loss of water. Desiccation tolerance requires an interplay of multiple events, including preserving the protein function and membrane integrity, preventing and mitigating oxidative stress, maintaining certain level of energy required for cellular activities in the desiccated state. Many of these crucial processes can be controlled and modulated at the level of organelle morphology and dynamics. However, little is understood about what organelle perturbations manifest in desiccation-sensitive cells as a consequence of drying or how this differs from organelle biology in desiccation-tolerant organisms undergoing anhydrobiosis. Results In this study, electron and optical microscopy was used to examine the dynamic changes of yeast cells during the desiccation process. Dramatic structural changes were observed during the desiccation process, including the diminishing of vacuoles, decrease of lipid droplets, decrease in mitochondrial cristae and increase of ER membrane, which is likely caused by ER stress and unfolded protein response. The survival rate was significantly decreased in mutants that are defective in lipid droplet biosynthesis, or cells treated with cerulenin, an inhibitor of fatty acid synthesis. Conclusion Our study suggests that the metabolism of lipid droplets and membrane may play an important role in yeast desiccation tolerance by providing cells with energy and possibly metabolic water. Additionally, the decrease in mitochondrial cristae coupled with a decrease in lipid droplets is indicative of a cellular response to reduce the production of reactive oxygen species.
- Subjects :
- Microbiology (medical)
Desiccation tolerance
Acclimatization
lcsh:QR1-502
Vacuole
Saccharomyces cerevisiae
Biology
Mitochondrion
Endoplasmic Reticulum
Microbiology
lcsh:Microbiology
03 medical and health sciences
0302 clinical medicine
Stress, Physiological
Lipid droplet
Organelle
Desiccation
030304 developmental biology
0303 health sciences
Endoplasmic reticulum
Cell Membrane
Lipid metabolism
Lipid Droplets
Anhydrobiosis
Endoplasmic Reticulum Stress
Lipid Metabolism
Cell biology
Mitochondria
Vacuoles
Unfolded protein response
Unfolded Protein Response
030217 neurology & neurosurgery
Research Article
Subjects
Details
- Language :
- English
- ISSN :
- 14712180
- Volume :
- 20
- Database :
- OpenAIRE
- Journal :
- BMC Microbiology
- Accession number :
- edsair.doi.dedup.....9c21f6a100a48912905e3ac15eabba52