Back to Search
Start Over
Variation in the N-terminal sequence of heavy chains of immunoglobulin G from rabbits of different allotype
- Source :
- The Biochemical journal. 112(2)
- Publication Year :
- 1969
-
Abstract
- The sequences of the N-terminal peptides prepared by Pronase digestion of the heavy chain of rabbit immunoglobulin G of allotype Aa1, Aa2 and Aa3 were determined and were shown to be related to the allotype. An N-terminal fragment of about 34 residues was also prepared from the allotype heavy chains, by cleavage with cyanogen bromide; the yield varied with the allotype. The sequences of the cyanogen bromide fragments from the Aa1 and Aa3 heavy chains contain allotype-related variations similar to those found in the N-terminal Pronase peptides, and these sequences are thought to be representative of the whole heavy-chain populations. There is about 60% homology between the two sequences, and superimposed on the differences between them there are a number of positions within each sequence at which at least two amino acids are present.
- Subjects :
- Electrophoresis
Paper
History
Chemical Phenomena
Genotype
Stereochemistry
Pronase
Homology (biology)
Immunoglobulin G
Education
chemistry.chemical_compound
Animals
Chymotrypsin
Trypsin
Amino Acid Sequence
Amino Acids
Peptide sequence
chemistry.chemical_classification
Autoanalysis
Cyanides
biology
Chemistry
Genetic Variation
Articles
Bromine
Allotype
Pepsin A
Computer Science Applications
Amino acid
Biochemistry
biology.protein
Chromatography, Gel
Cyanogen bromide
Rabbits
Peptides
Peptide Hydrolases
Subjects
Details
- ISSN :
- 02646021
- Volume :
- 112
- Issue :
- 2
- Database :
- OpenAIRE
- Journal :
- The Biochemical journal
- Accession number :
- edsair.doi.dedup.....9c098f3a25c7d30874da31bcb6cdfeb1