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Application of real-time polymerase chain reaction in detection of Entamoeba histolytica in pus aspirates of liver abscess patients
- Source :
- Foodborne Pathogens and Disease, 7(6), 637-641
- Publication Year :
- 2010
-
Abstract
- Entamoeba histolytica is the second major cause of liver abscess disease in humans, particularly in developing countries. Recently, DNA molecular-based methods have been employed to enhance the detection of E. histolytica in either pus or stool specimens. In this study, the results of real-time polymerase chain reaction (PCR) to detect E. histolytica DNA in pus from liver abscess cases were compared with those of indirect hemagglutination assay on the corresponding serum samples. Bacterial cultures were also performed on the pus samples for the diagnosis of pyogenic liver abscess. The real-time PCR detected E. histolytica DNA in 23 of 30 (76.7) pus samples, when compared with 14 of 30 (46.7) serum samples in which anti-Entamoeba antibodies were detected by indirect hemagglutination assay and 4 of 30 (13.3) pus samples that showed bacterial infection by culture. The use of real-time PCR is a promising detection method for diagnosis and epidemiology assessment of amoebic liver abscess. This record was migrated from the OpenDepot repository service in June, 2017 before shutting down.
- Subjects :
- Microbiological culture
Time Factors
Antibodies, Protozoan
Applied Microbiology and Biotechnology
Microbiology
Polymerase Chain Reaction
Sensitivity and Specificity
law.invention
Diagnosis, Differential
Entamoeba histolytica
fluids and secretions
law
Limit of Detection
parasitic diseases
medicine
Humans
Amoebiasis
Polymerase chain reaction
Pyogenic liver abscess
Amoebic liver abscess
Suppuration
biology
Entamoebiasis
Hemagglutination Tests
DNA, Protozoan
medicine.disease
biology.organism_classification
bacterial infections and mycoses
Virology
Bacterial Typing Techniques
Real-time polymerase chain reaction
Liver Abscess, Pyogenic
Liver Abscess, Amebic
diagnosis amebiasis pcr antigen samples assay ala
Animal Science and Zoology
Food Science
Liver abscess
Subjects
Details
- ISSN :
- 15567125
- Volume :
- 7
- Issue :
- 6
- Database :
- OpenAIRE
- Journal :
- Foodborne pathogens and disease
- Accession number :
- edsair.doi.dedup.....9c08f65b89b7c28d292a4e9d3bdb8d17