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Capabilities of Gabor-domain optical coherence microscopy for the assessment of corneal disease
- Source :
- Journal of Biomedical Optics, Journal of Biomedical Optics, Society of Photo-optical Instrumentation Engineers, 2019, 24 (04), pp.1. ⟨10.1117/1.jbo.24.4.046002⟩
- Publication Year :
- 2019
- Publisher :
- HAL CCSD, 2019.
-
Abstract
- To identify the microstructural modification of the corneal layers during the course of the disease, optical technologies have been pushing the boundary of innovation to achieve cellular resolution of deep layers of the cornea. Gabor-domain optical coherence microscopy (GD-OCM), an optical coherence tomography-based technique that can achieve an isotropic of ∼2-μm resolution over a volume of 1 mm×1 mm×1.2 mm, was developed to investigate the microstructural modifications of corneal layers in four common corneal diseases. Since individual layer visualization without cutting through several layers is challenging due to corneal curvature, a flattening algorithm was developed to remove the global curvature of the endothelial layer and display the full view of the endothelium and Descemet’s membrane in single en face images. As a result, GD-OCM revealed the qualitative changes in size and reflectivity of keratocytes in Fuchs endothelial corneal dystrophy (FECD), which varied by the degree of disease. More importantly, elongated shape and hyperactivation characteristics of keratocytes, associated with the early development of guttae, appeared to start in the posterior stroma very early in the disease process and move toward the anterior stroma during disease progression. This work opens a venue into the pathogenesis of FECD.
- Subjects :
- Paper
Male
Materials science
genetic structures
Biomedical Engineering
Diagnostic Techniques, Ophthalmological
Curvature
01 natural sciences
corneal disease
Imaging
Corneal Diseases
Cornea
010309 optics
Biomaterials
Optical coherence tomography
0103 physical sciences
Microscopy
medicine
Humans
corneal imaging
Image resolution
ComputingMilieux_MISCELLANEOUS
Aged, 80 and over
[PHYS.PHYS.PHYS-OPTICS]Physics [physics]/Physics [physics]/Optics [physics.optics]
optical coherence tomography
medicine.diagnostic_test
Fuchs' Endothelial Dystrophy
Fuchs endothelial corneal dystrophy
Equipment Design
Middle Aged
eye diseases
Atomic and Molecular Physics, and Optics
Electronic, Optical and Magnetic Materials
ophthalmology
medicine.anatomical_structure
Optical coherence microscopy
sense organs
Algorithms
Tomography, Optical Coherence
Biomedical engineering
Corneal disease
Subjects
Details
- Language :
- English
- ISSN :
- 10833668
- Database :
- OpenAIRE
- Journal :
- Journal of Biomedical Optics, Journal of Biomedical Optics, Society of Photo-optical Instrumentation Engineers, 2019, 24 (04), pp.1. ⟨10.1117/1.jbo.24.4.046002⟩
- Accession number :
- edsair.doi.dedup.....9bf7f4573f9d5a6547c6df2b1823fd7b