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Thermoresponsive Magnetic Nano-Biosensors for Rapid Measurements of Inorganic Arsenic and Cadmium
- Source :
- Sensors, Vol 12, Iss 10, Pp 14041-14052 (2012), Sensors; Volume 12; Issue 10; Pages: 14041-14052, Sensors (Basel, Switzerland)
- Publication Year :
- 2012
- Publisher :
- MDPI AG, 2012.
-
Abstract
- Green fluorescent protein-tagged sensor proteins, ArsR-GFP and CadC-GFP, have been produced as biosensors for simple and low-cost quantification of As(III) or Cd(II). In this study, the sensor protein-promoter DNA complexes were reconstructed on the surfaces of magnetic particles of different sizes. After the surface modification all the particles could be attracted by magnets, and released different amounts of GFP-tagged protein, according to the metal concentrations within 5 min, which caused significant increases in fluorescence. A detection limit of 1 µg/L for As(III) and Cd(II) in purified water was obtained only with the nanoparticles exhibiting enough magnetization after heat treatment for 1 min. Therefore, thermoresponsive magnetic nano-biosensors offer great advantages of rapidity and sensitivity for the measurement of the toxic metals in drinking water.
- Subjects :
- magnetic nanoparticles
Time Factors
Analytical chemistry
chemistry.chemical_element
Nanoparticle
Biosensing Techniques
biosensor
GFP
lcsh:Chemical technology
Biochemistry
Article
Arsenic
Analytical Chemistry
heavy metal
ArsR
CadC
lcsh:TP1-1185
Electrical and Electronic Engineering
Instrumentation
Detection limit
Cadmium
Drinking Water
Temperature
Flocculation
Purified water
Atomic and Molecular Physics, and Optics
Nanostructures
chemistry
Magnets
Surface modification
Magnetic nanoparticles
Biosensor
human activities
Water Pollutants, Chemical
Environmental Monitoring
Nuclear chemistry
Subjects
Details
- Language :
- English
- ISSN :
- 14248220
- Volume :
- 12
- Issue :
- 10
- Database :
- OpenAIRE
- Journal :
- Sensors
- Accession number :
- edsair.doi.dedup.....9bf03d7a5321c48b79605047bb0dc220