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An in vitro study of immunomodulatory effects of some saponins
- Source :
- International Journal of Immunopharmacology. 9:675-683
- Publication Year :
- 1987
- Publisher :
- Elsevier BV, 1987.
-
Abstract
- The in vitro immunomodulatory activities of a number of saponins (crude Quillaja saponin, Quillayanin, Quil-A and glycyrrhizic acid) are described. Addition of these saponin preparations to mouse spleen cell cultures resulted in significant cell proliferation. B-cells were induced to proliferate in the presence of the crude saponin, and T-cells in the presence of Quil-A. On the other hand, Quillayanin and glycyrrhizic acid stimulated both T- and B-lymphocytes equally. The selective proliferation of subtypes of lymphocytes correlated with restimulation responses by polyclonal mitogens. Pretreatment by lymphocytes with crude saponins induced significant T-cell responses to PHA and Con A, and to T-independent B-cell stimulation by LPS. Pulse exposure of spleen cells to Quil-A resulted in enhanced cell proliferation when restimulated with PHA, Con A and PWM. In comparison, similar exposure of lymphocytes to Quillayanin or glycyrrhizic acid produced markedly increased responses to PHA, Con A, PWM and LPS. Incubation of lymphocytes in the presence of Quillaja saponins and Quillayanin caused effector cell generation as determined in a one-way mixed lymphocyte reaction. In the case of lymphocytes cultured in the presence of crude saponins or glycyrrhizic acid, the supernatants contained active soluble factors. This was demonstrated by the observation that the addition of supernatants to spleen cell cultures induced spontaneous cell proliferation, and also amplified their responses to a suboptimal dose of PHA. The experimental data suggest that different components in the Quillaja saponin preparations may have selective effects on various subtypes of cell populations. Glycyrrhizic acid has the most profound immunomodulatory activity in vitro.(ABSTRACT TRUNCATED AT 250 WORDS)
- Subjects :
- T-Lymphocytes
Immunology
Cell
Saponin
In Vitro Techniques
Biology
Lymphocyte Activation
Quillaja Saponins
Mice
Anti-Infective Agents
medicine
Animals
Growth Substances
Pharmacology
chemistry.chemical_classification
B-Lymphocytes
Mice, Inbred BALB C
Cell growth
Quillaja
Biological activity
T lymphocyte
Saponins
Glycyrrhizic Acid
Mixed lymphocyte reaction
biology.organism_classification
Molecular biology
medicine.anatomical_structure
chemistry
Biochemistry
Cell culture
Glycyrrhetinic Acid
Female
Spleen
Subjects
Details
- ISSN :
- 01920561
- Volume :
- 9
- Database :
- OpenAIRE
- Journal :
- International Journal of Immunopharmacology
- Accession number :
- edsair.doi.dedup.....9bec1cb6cd5228e9cf30a920343f8ed6