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Single-cell RNA sequencing reveals a dynamic stromal niche within the evolving tumour microenvironment

Authors :
Jhuma Pramanik
Bidesh Mahata
Tzachi Hagai
Xi Chen
Sarah A. Teichmann
Jacqueline D. Shields
Roser Vento-Tormo
Muzlifah Haniffa
Gozde Kar
Sarah Davidson
Mirjana Efremova
Angela Riedel
Jani Huuhtanen
Publication Year :
2018
Publisher :
Cold Spring Harbor Laboratory, 2018.

Abstract

SummaryNon-cancerous stromal cells represent a highly diverse compartment of the tumour, yet their role across tumour evolution remains unclear. We employed single-cell RNA sequencing to determine stromal adaptations in murine melanoma at different points of tumour development. Naive lymphocytes recruited from lymph nodes underwent activation and clonal expansion within the tumour, prior to PD1 and Lag3 expression, while tumour-associated myeloid cells promoted the formation of a suppressive niche through cytokine secretion and inhibitory T cell interactions. We identified three temporally distinct cancer-associated fibroblast (CAF) populations displaying unique signatures, and verified these in human datasets. In early tumours, immune CXCL12/CSF1 and complement-expressing CAFs supported recruitment of macrophages, whereas contractile CAFs became more prevalent in later tumours. This study highlights the complex interplay and increasing diversity among cells that co-evolve with the tumour, indicating that from early stages of development, stromal cells acquire the capacity to modulate the immune landscape towards suppression.

Details

Database :
OpenAIRE
Accession number :
edsair.doi.dedup.....9be8455168de2adb687c8e721f893124
Full Text :
https://doi.org/10.1101/467225