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Adequate concentration of B cell leukemia/lymphoma 3 (Bcl3) is required for pluripotency and self-renewal of mouse embryonic stem cells via downregulation of Nanog transcription
- Source :
- BMB Reports
- Publication Year :
- 2018
- Publisher :
- Korean Society for Biochemistry and Molecular Biology, 2018.
-
Abstract
- B cell leukemia/lymphoma 3 (Bcl3) plays a pivotal role in immune homeostasis, cellular proliferation, and cell survival, as a co-activator or co-repressor of transcription of the NF-κB family. Recently, it was reported that Bcl3 positively regulates pluripotency genes, including Oct4, in mouse embryonic stem cells (mESCs). However, the role of Bcl3 in the maintenance of pluripotency and self-renewal activity is not fully established. Here, we report the dynamic regulation of the proliferation, pluripotency, and self-renewal of mESCs by Bcl3 via an influence on Nanog transcriptional activity. Bcl3 expression is predominantly observed in immature mESCs, but significantly decreased during cell differentiation by LIF depletion and in mESC-derived EBs. Importantly, the knockdown of Bcl3 resulted in the loss of self-renewal ability and decreased cell proliferation. Similarly, the ectopic expression of Bcl3 also resulted in a significant reduction of proliferation, and the self-renewal of mESCs was demonstrated by alkaline phosphatase staining and clonogenic single cell-derived colony assay. We further examined that Bcl3-mediated regulation of Nanog transcriptional activity in mESCs, which indicated that Bcl3 acts as a transcriptional repressor of Nanog expression in mESCs. In conclusion, we demonstrated that a sufficient concentration of Bcl3 in mESCs plays a critical role in the maintenance of pluripotency and the self-renewal of mESCs via the regulation of Nanog transcriptional activity. [BMB Reports 2018; 51(2): 92-97].
- Subjects :
- 0301 basic medicine
Homeobox protein NANOG
Regulation of gene expression
Bcl3
Mouse embryonic stem cell
Cell growth
Cellular differentiation
Nanog Homeobox Protein
General Medicine
Articles
Biology
Biochemistry
Embryonic stem cell
Nanog
Cell biology
03 medical and health sciences
030104 developmental biology
Self-renewal
Ectopic expression
Induced pluripotent stem cell
Molecular Biology
Subjects
Details
- Language :
- English
- ISSN :
- 1976670X and 19766696
- Volume :
- 51
- Issue :
- 2
- Database :
- OpenAIRE
- Journal :
- BMB Reports
- Accession number :
- edsair.doi.dedup.....9bdfdc0987a2bfa9fe78ad31f05a19a3