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3D Printed Polycaprolactone Carbon Nanotube Composite Scaffolds for Cardiac Tissue Engineering
- Source :
- Macromolecular bioscience. 17(4)
- Publication Year :
- 2016
-
Abstract
- Fabrication of tissue engineering scaffolds with the use of novel 3D printing has gained lot of attention, however systematic investigation of biomaterials for 3D printing have not been widely explored. In this report, well-defined structures of polycaprolactone (PCL) and PCL- carbon nanotube (PCL-CNT) composite scaffolds have been designed and fabricated using a 3D printer. Conditions for 3D printing has been optimized while the effects of varying CNT percentages with PCL matrix on the thermal, mechanical and biological properties of the printed scaffolds are studied. Raman spectroscopy is used to characterise the functionalized CNTs and its interactions with PCL matrix. Mechanical properties of the composites are characterised using nanoindentation. Maximum peak load, elastic modulus and hardness increases with increasing CNT content. Differential scanning calorimetry (DSC) studies reveal the thermal and crystalline behaviour of PCL and its CNT composites. Biodegradation studies are performed in Pseudomonas Lipase enzymatic media, showing its specificity and effect on degradation rate. Cell imaging and viability studies of H9c2 cells from rat origin on the scaffolds are performed using fluorescence imaging and MTT assay, respectively. PCL and its CNT composites are able to show cell proliferation and have the potential to be used in cardiac tissue engineering.
- Subjects :
- Materials science
Polymers and Plastics
Polyesters
Composite number
Bioengineering
macromolecular substances
02 engineering and technology
Carbon nanotube
010402 general chemistry
Spectrum Analysis, Raman
01 natural sciences
law.invention
Nanocomposites
Biomaterials
Myoblasts
chemistry.chemical_compound
Differential scanning calorimetry
Tissue engineering
law
Materials Testing
Materials Chemistry
Animals
Composite material
chemistry.chemical_classification
Nanocomposite
Calorimetry, Differential Scanning
Cell Death
Tissue Engineering
Tissue Scaffolds
Nanotubes, Carbon
Optical Imaging
technology, industry, and agriculture
Electric Conductivity
Temperature
Heart
Polymer
Lipase
Nanoindentation
musculoskeletal system
021001 nanoscience & nanotechnology
0104 chemical sciences
Rats
chemistry
Microscopy, Fluorescence
Polycaprolactone
Printing, Three-Dimensional
0210 nano-technology
Biotechnology
Subjects
Details
- ISSN :
- 16165195
- Volume :
- 17
- Issue :
- 4
- Database :
- OpenAIRE
- Journal :
- Macromolecular bioscience
- Accession number :
- edsair.doi.dedup.....9bd9a2bb3417797a2a9f8c43487947aa