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Overexpression of snakin‐1 gene enhances resistance to Rhizoctonia solani and Erwinia carotovora in transgenic potato plants
- Source :
- Molecular plant pathology 9 (3) : 329-338. (Mayo 2008), INTA Digital (INTA), Instituto Nacional de Tecnología Agropecuaria, instacron:INTA
- Publication Year :
- 2008
- Publisher :
- Wiley, 2008.
-
Abstract
- Snakin‐1 (SN1), a cysteine‐rich peptide with broad‐spectrum antimicrobial activity in vitro, was evaluated for its ability to confer resistance to pathogens in transgenic potatoes. Genetic variants of this gene were cloned from wild and cultivated Solanum species. Nucleotide sequences revealed highly evolutionary conservation with 91–98% identity values. Potato plants (S. tuberosum subsp. tuberosum cv. Kennebec) were transformed via Agrobacterium tumefaciens with a construct encoding the S. chacoense SN1 gene under the regulation of the ubiquitous CaMV 35S promoter. Transgenic lines were molecularly characterized and challenged with either Rhizoctonia solani or Erwinia carotovora to analyse whether constitutive in vivo overexpression of the SN1 gene may lead to disease resistance. Only transgenic lines that accumulated high levels of SN1 mRNA exhibited significant symptom reductions of R. solani infection such as stem cankers and damping‐off. Furthermore, these overexpressing lines showed significantly higher survival rates throughout the fungal resistance bioassays. In addition, the same lines showed significant protection against E. carotovora measured as: a reduction of lesion areas (from 46.5 to 88.1% with respect to the wild‐type), number of fallen leaves and thickened or necrotic stems. Enhanced resistance to these two important potato pathogens suggests in vivo antifungal and antibacterial activity of SN1 and thus its possible biotechnological application. Instituto de Biotecnología Fil: Almasia, Natalia Ines. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina. Fil: Bazzini, Ariel Alejandro. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina Fil: Hopp, Horacio Esteban. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina. Fil: Vazquez Rovere, Cecilia. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina Fil: Almasia, Natalia Ines. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Fisiología, Biología Molecular y Celular. Área de Biotecnología; Argentina Fil: Hopp, Horacio Esteban. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Fisiología, Biología Molecular y Celular. Área de Biotecnología; Argentina
- Subjects :
- Transgene
Molecular Sequence Data
Soil Science
Plant Science
Genetically modified crops
Plant disease resistance
Erwinia
Solanum
Plantas Transgénicas
Rhizoctonia
Microbiology
Rhizoctonia solani
Erwinia Carotovora
Potatoes
Transgenic Plants
Molecular Biology
Disease Resistance
Plant Diseases
Plant Proteins
Solanum tuberosum
Rhizoctonia Solani
biology
Pectobacterium Carotovorum
food and beverages
Original Articles
Agrobacterium tumefaciens
Plants, Genetically Modified
biology.organism_classification
Immunity, Innate
Pectobacterium carotovorum
Resistencia a la Enfermedad
Papa
Agronomy and Crop Science
Solanaceae
Subjects
Details
- ISSN :
- 13643703 and 14646722
- Volume :
- 9
- Database :
- OpenAIRE
- Journal :
- Molecular Plant Pathology
- Accession number :
- edsair.doi.dedup.....9b9949f2d44a6ecf407c2d8d9f6d6403