Back to Search
Start Over
The effect of drugs on cell structure of Tritrichomonas foetus
The effect of drugs on cell structure of Tritrichomonas foetus
- Source :
- Parasitology Research. 92:159-170
- Publication Year :
- 2004
- Publisher :
- Springer Science and Business Media LLC, 2004.
-
Abstract
- The effects of the microtubule affecting drugs taxol, nocodazole and colchicine on the cell cycle and ultrastructure of Tritrichomonas foetus, a protist parasite of cattle, were studied. Alterations in the cytoskeleton, motility and organellar ultrastructure were followed using anti-tubulin antibodies and fluorescence microscopy, scanning- and transmission-electron microscopy. Flow cytometry was also used to analyze the effect of the drugs on the cell cycle. T. foetus was treated with 10 microM taxol, 15 microM nocodazole or 1.5 mM colchicine for 12 h. The first effect observed was pseudocyst formation and alterations in cell motility. The cell cycle was affected and the cells have blocked cytokinesis, but not karyokinesis. The behavior of Golgi, hydrogenosomes and vacuoles was analyzed. The following effects were seen following drug treatments: (1) cell motility was altered and flagella internalized; (2) microtubules of the pelta-axostyle complex were not depolymerized and the axostyle assumed a curved form; (3) hydrogenosomes were of abnormal size and shape; (4) cells became multinucleate; (5) the division process was blocked in cytokinesis; (6) autophagic vacuoles containing a large amount of microtubules were seen; (7) axoneme organization was altered; (8) zoids were formed; (9) signs of cell death, such as membrane blebbing, were observed.
- Subjects :
- Paclitaxel
Tritrichomonas foetus
Microtubules
chemistry.chemical_compound
Multinucleate
Animals
Axostyle
Mitosis
Protozoan Infections
General Veterinary
biology
Nocodazole
Cell Cycle
General Medicine
Cell cycle
Flow Cytometry
biology.organism_classification
Cell biology
Microscopy, Electron
Infectious Diseases
chemistry
Insect Science
Ultrastructure
Parasitology
Colchicine
Cytokinesis
Subjects
Details
- ISSN :
- 14321955 and 09320113
- Volume :
- 92
- Database :
- OpenAIRE
- Journal :
- Parasitology Research
- Accession number :
- edsair.doi.dedup.....9b96eb8d04d97fed500764f4c3b66dfa
- Full Text :
- https://doi.org/10.1007/s00436-003-1023-2