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Biodistribution of Small Interfering RNA at the Organ and Cellular Levels after Lipid Nanoparticle-mediated Delivery
- Source :
- Journal of Histochemistry & Cytochemistry. 59:727-740
- Publication Year :
- 2011
- Publisher :
- SAGE Publications, 2011.
-
Abstract
- Chemically stabilized small interfering RNA (siRNA) can be delivered systemically by intravenous injection of lipid nanoparticles (LNPs) in rodents and primates. The biodistribution and kinetics of LNP–siRNA delivery in mice at organ and cellular resolution have been studied using immunofluorescence (IF) staining and quantitative polymerase chain reaction (qPCR). At 0.5 and 2 hr post tail vein injection of Cy5-labeled siRNA encapsulated in LNP, the organ rank-order of siRNA levels is liver > spleen > kidney, with only negligible accumulation in duodenum, lung, heart, and brain. Similar conclusions were drawn by using qPCR to measure tissue siRNA levels as a secondary end point. siRNA levels in these tissues decreased by more than 10-fold after 24 hr. Within the liver, LNPs delivered siRNA to hepatocytes, Kupffer cells, and sinusoids in a time-dependent manner, as revealed by IF staining and signal quantitation methods established using OPERA/Columbus software. siRNA first accumulated in liver sinusoids and trafficked to hepatocytes by 2 hr post dose, corresponding to the onset of target mRNA silencing. Fluorescence in situ hybridization methods were used to detect both strands of siRNA in fixed tissues. Collectively, the authors have implemented a platform to evaluate biodistribution of siRNA across cell types and across tissues in vivo, with the objective of elucidating the pharmacokinetic and pharmacodynamic relationship to guide optimization of delivery vehicles.
- Subjects :
- Small interfering RNA
Biodistribution
Histology
Fluorescent Antibody Technique
In situ hybridization
Biology
Autoantigens
Article
Mice
In vivo
medicine
Animals
Gene silencing
Tissue Distribution
RNA, Messenger
RNA, Small Interfering
In Situ Hybridization, Fluorescence
Drug Carriers
Kupffer cell
RNA
Lipids
Molecular biology
medicine.anatomical_structure
Ribonucleoproteins
Gene Knockdown Techniques
Hepatocyte
Nanoparticles
Anatomy
Subjects
Details
- ISSN :
- 15515044 and 00221554
- Volume :
- 59
- Database :
- OpenAIRE
- Journal :
- Journal of Histochemistry & Cytochemistry
- Accession number :
- edsair.doi.dedup.....9b8fb9589171de40eeb1680b0c58a8ff