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Simple gas chromatographic method for the quantification of total cholesterol in fish meats
- Source :
- Syed Tufail Hussain Sherazi
- Publication Year :
- 2010
-
Abstract
- Six fish speciesCirrhinus mrigala (Morakhi), Labeo rohita (Danbhro), Catla catla (Thalli), Wallago attu (Jarko), Ctenopharyngodon idella (Grass), and Cyprimus carpio (Gulfam)commonly found in the Pakistani Indus river, were selected for fat and cholesterol evaluation. Fat content was quantified by the Folch method and cholesterol content was determined by a simple GC method. The application of the proposed method to quantify cholesterol content revealed variations among the six breeds evaluated. The breeds studied contained 0.801.95 fat, indicating that all investigated fishes were lean; significant variation was found in cholesterol content, and ranged between 72 and 392 mg/100 g. Standard addition analyses showed that the method was accurate, as the recovery of cholesterol varied from 95 to 103, with a coefficient of variance not more than 5.6. The results showed that, after the development of suitable calibration, within 11 min, the cholesterol could be accurately determined by GC.
- Subjects :
- Pharmacology
Chromatography
Chromatography, Gas
biology
Cholesterol
Coefficient of variation
Fishes
biology.organism_classification
Catla
Analytical Chemistry
Labeo
chemistry.chemical_compound
chemistry
Seafood
Standard addition
Environmental Chemistry
Animals
Gas chromatography
Agronomy and Crop Science
Wallago attu
Quantitative analysis (chemistry)
Food Science
Subjects
Details
- ISSN :
- 10603271
- Volume :
- 93
- Issue :
- 4
- Database :
- OpenAIRE
- Journal :
- Journal of AOAC International
- Accession number :
- edsair.doi.dedup.....9b8bea730f57a14f1493ea494786cf84