Additional file 4 of Reduced and highly diverse peripheral HIV-1 reservoir in virally suppressed patients infected with non-B HIV-1 strains in Uganda

Additional file 4: Figure S3. Analytical sensitivity of the EDITS assay. Jurkat E4, a cell line latently infected with a single copy of HIV-1 per cell, and Jurkat E6-1, an HIV-negative human T cell lymphoblast, were quantified and serial dilutions were used to prepare ten mixtures containing 0 to 400 Jurkat E4 cells in a background of one million Jurkat E6-1 cells in eight biological replicates. Cell mixtures were activated and cell-associated spliced HIV-1 RNA and proviral DNA quantified using EDITS assay as described in ���Methods��� section. Heatmaps indicate the number of HIV-1 vpu/end reads in each of the eight cell mixture replicates. Linear dynamic ranges and regression values (Pearson���s coefficient correlation) describing the relationship between mapped vpu/env reads and number of HIV positive cells in the mixtures are indicated. Median mapped reads and interquartile range are depicted.r, correlation coeficient; p, two-tailed p value.