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Expression of Chemokine Genes in Murine Macrophages Infected withOrientia tsutsugamushi
- Source :
- Infection and Immunity. 68:594-602
- Publication Year :
- 2000
- Publisher :
- American Society for Microbiology, 2000.
-
Abstract
- Scrub typhus, caused byOrientia tsutsugamushiinfection, is characterized by local as well as systemic inflammatory manifestations. Inflammation is initiated byO. tsutsugamushi-infected macrophages and endothelial cells in the dermis. We investigated the regulation of chemokine induction in macrophage cell line J774A.1 in response toO. tsutsugamushiinfection. The mRNAs for macrophage inflammatory proteins 1α/β (MIP-1α/β), MIP-2, and macrophage chemoattractant protein 1 were induced within 30 min, and their levels showed a transitory peak for 3 to 12 h. However, the lymphotactin, eotaxin, gamma interferon-inducible protein 10, and T-cell activation gene 3 mRNAs were not detected by RNase protection assays. Heat-killedO. tsutsugamushiinduced a similar extent of chemokine responses. Induction of the chemokine genes was not blocked by the eukaryotic protein synthesis inhibitor cycloheximide, suggesting that de novo synthesis of host cell protein is not required for these transcriptional responses. The induction of chemokine mRNAs byO. tsutsugamushiwas blocked by the inhibitors of NF-κB activation. Furthermore,O. tsutsugamushiinduced the nuclear translocation and activation of NF-κB. These results demonstrate that heat-stable molecules ofO. tsutsugamushiinduce a subset of chemokine genes and that induction involves activation of the transcription factor NF-κB.
- Subjects :
- CCR2
Hot Temperature
Immunology
C-C chemokine receptor type 6
Biology
Microbiology
Cell Line
Mice
Chemokine receptor
Animals
CXCL10
RNA, Messenger
Host Response and Inflammation
Reverse Transcriptase Polymerase Chain Reaction
Macrophages
Tosylphenylalanyl Chloromethyl Ketone
NF-kappa B
bacterial infections and mycoses
Molecular biology
Orientia tsutsugamushi
CXCL2
Infectious Diseases
Gene Expression Regulation
CXCL9
Parasitology
Chemokines
CCL22
CCL21
Subjects
Details
- ISSN :
- 10985522 and 00199567
- Volume :
- 68
- Database :
- OpenAIRE
- Journal :
- Infection and Immunity
- Accession number :
- edsair.doi.dedup.....9b7b20ebba2aa550520a5067428772c3