Back to Search
Start Over
Sensitive detection of protein biomarkers using silver nanoparticles enhanced immunofluorescence assay
- Source :
- Theranostics
- Publication Year :
- 2017
- Publisher :
- Ivyspring International Publisher, 2017.
-
Abstract
- Detection of biomarkers is extremely important for the early diagnosis of diseases. Here, we developed an easy and highly sensitive fluorescence detection system for the determination of biomarkers by combining the rapid separation of magnetic beads and silver nanoparticles labeled antibodies. An ultrasensitive silver ions fluorescence probe 3', 6'-bis (diethylamino)-2-(2-iodoethyl) spiro[isoindoline-1, 9'-xanthen]-3-one (Ag+-FP) was applied to immunoassay. A significant signal amplification was achieved as the AgNPs can be dissolved by H2O2 and generate numerous Ag+, which would turn "on" the fluorescence of Ag+-FP. Using α-fetoprotein (AFP) and C-reactive protein (CRP) as target analytes, good linear responses were obtained from 0.1 to 10 ng mL-1 and the limits of detection (LOD) were as low as 70 pg·mL-1 and 30 pg·mL-1, respectively. In addition, the developed system was further evaluated for the detection of real samples including 30 positive serum specimens obtained from hepatocarcinoma patients and 20 negative serum samples, and performs as well as the commercial electrochemiluminescence immunoassay (ECLI) method with less cost and more convenience. Thus, the designed detection system can be used as a promising platform for the detection of a variety of biomarkers and served as a powerful tool in clinical diagnosis.
- Subjects :
- Analyte
silver nanoparticles
Silver
Protein biomarkers
Medicine (miscellaneous)
Fluorescent Antibody Technique
02 engineering and technology
010402 general chemistry
Immunofluorescence
01 natural sciences
Silver nanoparticle
magnetic beads
medicine
Electrochemiluminescence
Humans
Pharmacology, Toxicology and Pharmaceutics (miscellaneous)
Detection limit
Chromatography
medicine.diagnostic_test
Chemistry
Diagnostic Tests, Routine
Proteins
021001 nanoscience & nanotechnology
Fluorescence
0104 chemical sciences
biomarkers
Immunoassay
fluorescence detection
Nanoparticles
0210 nano-technology
Biomarkers
Research Paper
Subjects
Details
- Language :
- English
- ISSN :
- 18387640
- Volume :
- 7
- Issue :
- 4
- Database :
- OpenAIRE
- Journal :
- Theranostics
- Accession number :
- edsair.doi.dedup.....9b7817890ef5049e423db70d0e40498e