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Crystallographic studies of Homo sapiens ribosomal decoding A site complexed with aminoglycosides
- Source :
- Nucleic Acids Symposium Series, Nucleic Acids Symposium Series, Oxford University Press, 2005, 49, pp.253-254
- Publication Year :
- 2005
- Publisher :
- HAL CCSD, 2005.
-
Abstract
- Aminoglycosides are highly effective antibacterial drugs that decrease translation accuracy by binding to the aminoacyl-tRNA decoding site (A site) of 16S ribosomal RNA. On the other hand, they are highly toxic to mammals through kidney and ear-associated illnesses by binding to ribosomal A sites. To understand the mechanism of toxicity of aminoglycosides to mammals at atomic level, crystallographic studies have been carried out with a number of Homo sapiens mitochondrial and cytoplasmic A sites complexed with aminoglycosides. Several X-ray diffraction data sets were successfully collected. Initial phases of mitochondrial A site with tobramycin and cytoplasmic A site with paromomycin were derived by the molecular replacement method. Refinements of atomic parameters are now under progress.
- Subjects :
- Paromomycin
RNA, Mitochondrial
Mitochondrion
Biology
Crystallography, X-Ray
Ribosome
03 medical and health sciences
0302 clinical medicine
medicine
[SDV.BBM] Life Sciences [q-bio]/Biochemistry, Molecular Biology
Humans
Nebramycin
[SDV.BBM]Life Sciences [q-bio]/Biochemistry, Molecular Biology
Molecular replacement
030304 developmental biology
0303 health sciences
Translation (biology)
General Medicine
Ribosomal RNA
Anti-Bacterial Agents
3. Good health
A-site
Crystallography
Aminoglycosides
Biochemistry
RNA, Ribosomal
Homo sapiens
Tobramycin
RNA
030217 neurology & neurosurgery
medicine.drug
Subjects
Details
- Language :
- English
- ISSN :
- 02613166 and 17468272
- Database :
- OpenAIRE
- Journal :
- Nucleic Acids Symposium Series, Nucleic Acids Symposium Series, Oxford University Press, 2005, 49, pp.253-254
- Accession number :
- edsair.doi.dedup.....9b66058d6079a9ad03969f765d945db5