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11.7 T MR Imaging Revealed Dilatation of Virchow–Robin Spaces within Hippocampus in Maternally Lipopolysaccharide-exposed Rats
- Source :
- Magnetic Resonance in Medical Sciences
- Publication Year :
- 2017
- Publisher :
- Japanese Society for Magnetic Resonance in Medicine, 2017.
-
Abstract
- Purpose: 11.7 Tesla MRI was examined to detect Virchow–Robin spaces (VRSs) smaller than 100 μm in the rat brain. The effects of maternal exposure to lipopolysaccharide (LPS) were evaluated on basis of the number of dilated VRSs in the offspring rat brain. Methods: T2-weighted MRI with an in-plane resolution up to 78 μm (repetition time = 5000 ms, echo time = 35 ms, slice thickness = 250 μm, imaging plane, coronal) was applied to identify VRSs. The dilated VRSs were counted in the rat brain at 5 and 10 weeks of age. The dams of half the number in each group were treated with LPS during pregnancy, and the remaining half was employed as control. LPS injection in gestation period was used to simulate maternal infections, the method of which was widely accepted as a rat model inducing neuropsychiatric disorders in the offspring. Effects of LPS exposure on the offspring rat brain were statistically investigated. Results: VRSs as small as 78 μm were successfully detected by the ultra high-field MRI. All dilated VRSs were observed within lacunosum molecular layer of hippocampus, and molecular and granular layers of dentate gyrus around hippocampal fissure. In juvenile rats (5 weeks of age), the number of dilated VRSs was significantly increased in the prenatal LPS exposed rat brain (12.9 ± 2.4, n = 7) than in the control (5.3 ± 1.5, n = 7, P < 0.05), while in young adult rats (10 weeks of age), there was no significant difference in the number between the prenatal LPS exposed rat brain (3.6 ± 0.9, n = 5) and the control (2.6 ± 0.4, n = 5). Conclusion: The results of the present study suggested that maternal infection might cause dilatation of VRSs through neural damages especially in the dentate gyrus of the offspring rats. Thus, ultra high-field MRI can offer a promising diagnostic tool capable of determining the location of neonatal brain damage caused by maternal infections.
- Subjects :
- Lipopolysaccharides
Male
0301 basic medicine
Pathology
medicine.medical_specialty
ultra high-field T2-weighted MR imaging
Lipopolysaccharide
Offspring
Hippocampus
Virchow robin spaces
Subarachnoid Space
maternal infection
03 medical and health sciences
chemistry.chemical_compound
0302 clinical medicine
Pregnancy
medicine
Animals
Radiology, Nuclear Medicine and imaging
dentate gyrus
hippocampal damage
Rats, Wistar
dilated Virchow–Robin space
business.industry
Dentate gyrus
medicine.disease
Magnetic Resonance Imaging
Mr imaging
Rats
030104 developmental biology
chemistry
Hippocampal Fissure
Prenatal Exposure Delayed Effects
Female
business
Major Paper
030217 neurology & neurosurgery
Subjects
Details
- ISSN :
- 18802206 and 13473182
- Volume :
- 16
- Database :
- OpenAIRE
- Journal :
- Magnetic Resonance in Medical Sciences
- Accession number :
- edsair.doi.dedup.....9b623a7b95294285cbc0aaf4b3f17b70