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Metabolomics of the rat lens: a combined LC-MS and NMR study
- Source :
- Experimental eye research. 125
- Publication Year :
- 2014
-
Abstract
- This work is the first comprehensive report on the quantitative metabolomic composition of the rat lens. Quantitative metabolomic profiles of lenses were acquired with the combined use of high-frequency nuclear magnetic resonance (NMR) and high-performance liquid chromatography with high-resolution mass-spectrometric detection (LC-MS) methods. More than forty low molecular weight compounds found in the lens have been reliably identified and quantified. The most abundant metabolites in the 3-month-old Wistar rat lens are taurine, hypotaurine, lactate, phosphocholine and reduced glutathione. The analysis of age-related changes in the lens metabolomic composition shows a gradual decrease of the content of most metabolites. This decrease is the most pronounced between 1 and 3 months, which probably corresponds to the completion of the lens maturation in one-month-old rats and to the high rate of the young lens growth. The enhanced levels of tryptophan, tyrosine, carnitine, glycerophosphate, GSH and GSSG were found in lenses of senescence-accelerated OXYS rats; for some metabolites, this effect may probably be attributed to the compensatory response to oxidative stress.
- Subjects :
- Taurine
Aging
Magnetic Resonance Spectroscopy
Hypotaurine
Biology
medicine.disease_cause
Mass Spectrometry
Cellular and Molecular Neuroscience
chemistry.chemical_compound
Metabolomics
Liquid chromatography–mass spectrometry
Lens, Crystalline
medicine
Animals
Carnitine
Rats, Wistar
Eye Proteins
Phosphocholine
Chromatography
Aging, Premature
Glutathione
Sensory Systems
Ophthalmology
chemistry
Biochemistry
Oxidative stress
medicine.drug
Chromatography, Liquid
Subjects
Details
- ISSN :
- 10960007
- Volume :
- 125
- Database :
- OpenAIRE
- Journal :
- Experimental eye research
- Accession number :
- edsair.doi.dedup.....9b5666958127e8acb44483f1daef419c