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Nucleotide sequence of the kanamycin resistance determinant of plasmid RP4: Homology to other aminoglycoside 3′-phosphotransferases
- Source :
- Plasmid. 18:193-204
- Publication Year :
- 1987
- Publisher :
- Elsevier BV, 1987.
-
Abstract
- The kanamycin resistance determinant of the broad-host-range plasmid RP4 encodes an aminoglycoside 3'-phosphotransferase of type I. The nucleotide sequence of the kanamycin resistance gene (Kmr) and the right end of the insertion element IS8 of plasmid RP4 has been determined. The gene (816 bp) is located between IS8 and the region (Tra 1) encoding plasmid factors mediating bacterial conjugation. Kmr and Tra 1 are transcribed toward each other. The nucleotide sequence has been compared to five related aphA genes originating from gram-negative and gram-positive organisms and from antibiotic producers. Among these that of Tn903 shares the highest degree of similarity (60%) with the RP4 gene. Significant similarities were also detected between the amino acid sequences of the six enzymes. The C-terminal domains of six different aminoglycoside 3'-phosphotransferases (APH(3'] are highly conserved. They are substantially similar to segments of a variety of enzymes using ATP as cofactor. The role of the C-terminal sequences of APH(3') as potential domains for ATP recognition and binding is discussed.
- Subjects :
- Genetics
Kanamycin Resistance
Base Sequence
Kanamycin Kinase
R Factors
Kanamycin kinase
Bacterial conjugation
Molecular Sequence Data
Phosphotransferases
Aminoglycoside
Nucleic acid sequence
biochemical phenomena, metabolism, and nutrition
Biology
Molecular biology
Phosphotransferase
Plasmid
Sequence Homology, Nucleic Acid
DNA Transposable Elements
Cloning, Molecular
Molecular Biology
Gene
Subjects
Details
- ISSN :
- 0147619X
- Volume :
- 18
- Database :
- OpenAIRE
- Journal :
- Plasmid
- Accession number :
- edsair.doi.dedup.....9b3ef8548f8b2dd41f7459cf3387b7e8