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An ethoxylated surfactant enhances the penetration of the sulfated laminarin through leaf cuticle and stomata, leading to increased induced resistance against grapevine downy mildew
An ethoxylated surfactant enhances the penetration of the sulfated laminarin through leaf cuticle and stomata, leading to increased induced resistance against grapevine downy mildew
- Source :
- Physiologia Plantarum, Physiologia Plantarum, Wiley, 2016, 156 (3), pp.338-350. ⟨10.1111/ppl.12394⟩, Physiologia Plantarum, 2016, 156 (3), pp.338-350. 〈10.1111/ppl.12394〉, Physiologia Plantarum, 2016, 156 (3), pp.338-350. ⟨10.1111/ppl.12394⟩
- Publication Year :
- 2015
- Publisher :
- Wiley, 2015.
-
Abstract
- International audience; Some β-1,3-glucans and particularly sulfated laminarin (PS3) are known as resistance inducers (RIs) in grapevine against the downy mildew. However, their efficacy in vineyard is still often too low, which might be caused by a limited penetration through the leaf cuticle following spray application. We used (14) C-sucrose uptake experiments with grapevine leaves in order to select a surfactant as saccharide penetration enhancer. Our results showed that though sucrose foliar uptake was low, it was strongly enhanced by Dehscofix CO125 (DE), a highly ethoxylated surfactant. Fluorescent saccharides were then produced and laser scanning microscopy was used to analyze their foliar diffusion pattern in Arabidopsis thaliana and grapevine. Interestingly, sucrose and PS3 were seemingly able to penetrate the leaf cuticle only when formulated with DE. Diffusion could preferentially occur via stomata, anticlinal cell walls and trichomes. In grapevine, PS3 penetration rate was much higher on the stomateous abaxial surface of the leaf than on the adaxial surface. Finally, using DE allowed a higher level of downy mildew control by PS3, which corroborated diffusion observations. Our results have practical consequences for the improvement of treatments with saccharidic inducers on grape. That is, formulation of such RIs plays a critical role for their cuticular diffusion and consequently their efficacy. Also, spray application should preferentially target the abaxial surface of the leaves in order to maximize their penetration
- Subjects :
- Ethylene Oxide
0106 biological sciences
0301 basic medicine
Sucrose
Physiology
Plant Science
Biology
Disaccharides
[ CHIM ] Chemical Sciences
01 natural sciences
Fluorescence
Diffusion
Cell wall
Surface-Active Agents
03 medical and health sciences
Laminarin
chemistry.chemical_compound
Pulmonary surfactant
Polysaccharides
Botany
Genetics
[CHIM]Chemical Sciences
Vitis
Carbon Radioisotopes
Disease Resistance
Plant Diseases
Plant Stomata
food and beverages
Cell Biology
General Medicine
Penetration (firestop)
Trichome
Kinetics
Horticulture
Cholesterol
030104 developmental biology
Oomycetes
Plant cuticle
chemistry
Waxes
Downy mildew
010606 plant biology & botany
Subjects
Details
- ISSN :
- 00319317 and 13993054
- Volume :
- 156
- Database :
- OpenAIRE
- Journal :
- Physiologia Plantarum
- Accession number :
- edsair.doi.dedup.....9b30b81765fce3726899832833f21645
- Full Text :
- https://doi.org/10.1111/ppl.12394