Survey on proper and safe use of biological safety cabinets (BSCs) in research, bio-medical and animal laboratories in Karachi, Pakistan a cross sectional study

Introduction Past incidences have been recorded relating to mishandling of hazardous material either due to insufficient knowledge or inappropriate equipment resulting in the indirect transfer of infectious agents. It is necessary to identify gaps that inhibit proper protection of healthcare professionals from being inadvertently exposed to biological risks. Methods A cross sectional study was conducted at multiple laboratories across Karachi, Pakistan from January to August 2018. Descriptive questionnaire was used to record data about types and usage of Biological Safety Cabinets (BSCs), along with policies and practices adapted for its certification. Results A total of 30 laboratories were visited which included Clinical/Biomedical, University hospital, R&D and animal laboratories. Among these 60% always used BSCs, 30% periodically used BSCs while 10% did not have any BSCs. Among these facilities 63.6% had BSC class II-A2, 39.9% had BSC class II-B2, 4.5% had BSC class III and only 0.9% had BSC class I cabinet. No protocols for field certification of BSCs were available. Conclusion Establishment of biosafety training sessions, implementation and development of protocol for field certification for proper and safe use of BSCs on a local basis accompanied by continuous monitoring is required, for creating a safe environment for all laboratory staff.