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Rolling Circle Amplification as an Efficient Analytical Tool for Rapid Detection of Contaminants in Aqueous Environments
- Source :
- Biosensors, Vol 11, Iss 352, p 352 (2021), Biosensors
- Publication Year :
- 2021
- Publisher :
- MDPI AG, 2021.
-
Abstract
- Environmental contaminants are a global concern, and an effective strategy for remediation is to develop a rapid, on-site, and affordable monitoring method. However, this remains challenging, especially with regard to the detection of various contaminants in complex water environments. The application of molecular methods has recently attracted increasing attention; for example, rolling circle amplification (RCA) is an isothermal enzymatic process in which a short nucleic acid primer is amplified to form a long single-stranded nucleic acid using a circular template and special nucleic acid polymerases. Furthermore, this approach can be further engineered into a device for point-of-need monitoring of environmental pollutants. In this paper, we describe the fundamental principles of RCA and the advantages and disadvantages of RCA assays. Then, we discuss the recently developed RCA-based tools for environmental analysis to determine various targets, including heavy metals, organic small molecules, nucleic acids, peptides, proteins, and even microorganisms in aqueous environments. Finally, we summarize the challenges and outline strategies for the advancement of this technique for application in contaminant monitoring.
- Subjects :
- organic molecules
Environmental analysis
Computer science
Clinical Biochemistry
Heavy metals
General Medicine
Review
Biosensing Techniques
Contamination
Rapid detection
Rolling circle replication
Nucleic Acids
Environmental monitoring
Nucleic acid
Biochemical engineering
Monitoring methods
heavy metals
microorganisms
Nucleic Acid Amplification Techniques
Water Pollutants, Chemical
rolling circle amplification
TP248.13-248.65
environmental monitoring
Biotechnology
Subjects
Details
- Language :
- English
- ISSN :
- 20796374
- Volume :
- 11
- Issue :
- 352
- Database :
- OpenAIRE
- Journal :
- Biosensors
- Accession number :
- edsair.doi.dedup.....9afc3d91c9d538cb43c229f8b75632b2