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Jiedu Sangen Decoction Inhibits the Invasion and Metastasis of Colorectal Cancer Cells by Regulating EMT through the Hippo Signaling Pathway
- Source :
- Evidence-based Complementary and Alternative Medicine : eCAM, Evidence-Based Complementary and Alternative Medicine, Vol 2019 (2019)
- Publication Year :
- 2019
-
Abstract
- Colorectal cancer (CRC) is one of the most common malignant tumors affecting the digestive tract. Moreover, the invasion and metastasis of CRC are the main reason therapy is usually inefficient. Decreased intercellular adhesion and enhanced cell motility induced by epithelial-mesenchymal transition (EMT) provide the basic conditions for the invasion and metastasis of the epithelial tumor cells of CRC. The Jiedu Sangen Decoction (JSD) is a prescription that has been used for more than 50 years in the treatment of CRC in the Zhejiang Hospital of Traditional Chinese Medicine. The aim of this study was to investigate the mechanism of JSD-triggered inhibition of invasion and metastasis in colon cancer. In vitro, the EMT model of the SW480 cells was induced by using epithelial growth factor (50 ng/mL). In vivo, the murine model of liver metastasis was constructed by inoculating mice with the SW480 cells. The effects of JSD on cell migration, invasion, and proliferation were determined using the transwell assay and CCK-8 assay. Moreover, the proteins related to the EMT process and the Hippo signaling pathway in the cancerous tissues and cell lines were determined by western blotting and immunostaining. JSD could significantly inhibit the proliferation, migration, and invasion of CRC cells and reverse their EMT status (all, P < 0.05). Moreover, after intervention with JSD, the levels of E-Cadherin (E-cad) increased, whereas the expression levels of N-Cadherin (N-cad), Yes-associated protein (YAP), and the transcriptional coactivator with the PDZ-binding motif (TAZ) decreased in both the SW480 cells and the tumor tissues. In summary, JSD reversed EMT and inhibited the invasion and metastasis of CRC cells through the Hippo signaling pathway.
- Subjects :
- 0303 health sciences
Hippo signaling pathway
Article Subject
Colorectal cancer
Growth factor
medicine.medical_treatment
Motility
Cell migration
lcsh:Other systems of medicine
Biology
lcsh:RZ201-999
medicine.disease
digestive system diseases
Metastasis
Blot
03 medical and health sciences
0302 clinical medicine
Complementary and alternative medicine
Cell culture
030220 oncology & carcinogenesis
Cancer research
medicine
030304 developmental biology
Research Article
Subjects
Details
- ISSN :
- 1741427X
- Volume :
- 2019
- Database :
- OpenAIRE
- Journal :
- Evidence-based complementary and alternative medicine : eCAM
- Accession number :
- edsair.doi.dedup.....9af05bb266898c40a0b7cfb81c12e92c