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Boronate affinity-based photoactivatable magnetic nanoparticles for the oriented and irreversible conjugation of Fc-fused lectins and antibodies† †Electronic supplementary information (ESI) available. See DOI: 10.1039/c9sc01613a
- Source :
- Chemical Science
- Publication Year :
- 2019
- Publisher :
- Royal Society of Chemistry, 2019.
-
Abstract
- A combination of boronic acid and a photoactivatable diazirine enables oriented conjugation of Fc-fused lectins and antibodies on nanoparticles.<br />The utilization of immuno-magnetic nanoparticles (MNPs) for the selective capture, enrichment, and separation of specific glycoproteins from complicated biological samples is appealing for the discovery of disease biomarkers. Herein, MNPs were designed and anchored with abundant boronic acid (BA) and photoreactive alkyl diazirine (Diaz) functional groups to obtain permanently tethered Fc-fused Siglec-2 and antiserum amyloid A (SAA) mAb with the assistance of reversible boronate affinity and UV light activation in an orientation-controlled manner. The Siglec-2–Fc-functionalized MNPs showed excellent stability in fetal bovine serum (FBS) and excellent efficiency in the extraction of cell membrane glycoproteins. The anti-SAA mAb-functionalized MNPs maintained active Ab orientation and preserved antigen recognition capability in biological samples. Thus, the BA–Diaz-based strategy holds promise for the immobilization of glycoproteins, such as antibodies, with the original protein binding activity maintained, which can provide better enrichment for the sensitive detection of target proteins.
- Subjects :
- Antiserum
chemistry.chemical_classification
inorganic chemicals
010405 organic chemistry
medicine.drug_class
General Chemistry
Plasma protein binding
010402 general chemistry
Monoclonal antibody
01 natural sciences
0104 chemical sciences
Cell membrane
chemistry.chemical_compound
Chemistry
medicine.anatomical_structure
chemistry
Diazirine
medicine
Biophysics
Glycoprotein
Fetal bovine serum
Boronic acid
Subjects
Details
- Language :
- English
- ISSN :
- 20416539 and 20416520
- Volume :
- 10
- Issue :
- 37
- Database :
- OpenAIRE
- Journal :
- Chemical Science
- Accession number :
- edsair.doi.dedup.....9ad6d923c9a72103cb4ac216ef632e03