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A bioengineered drug-Eluting scaffold accelerated cutaneous wound healing In diabetic mice
- Source :
- Colloids and Surfaces B: Biointerfaces. 145:226-231
- Publication Year :
- 2016
- Publisher :
- Elsevier BV, 2016.
-
Abstract
- Hyperglycemia in diabetic patients can greatly hinder the wound healing process. In this study we investigated if the engagement of F4/80(+) murine macrophages could accelerate the cutaneous wound healing in streptozotocin induced diabetic mice. To facilitate the engagement of macrophages, we engineered a drug-eluting electrospun scaffold with a payload of monocyte chemoattractant protein-1 (MCP-1). MCP-1 could be readily released from the scaffold within 3 days. The electrospun scaffold showed no cytotoxic effects on human keratinocytes in vitro. Full-thickness excisional cutaneous wound was created in diabetic mice. The wound fully recovered within 10 days in mice treated with the drug-eluting scaffold. In contrast, the wound took 14 days to fully recover in control groups. The use of drug-eluting scaffold also improved the re-epithelialization. Furthermore, we observed a larger population of F4/80(+) macrophages in the wound bed of mice treated with drug-eluting scaffolds on day 3. This marked increase of macrophages in the wound bed could have contributed to the accelerated wound healing. Our study shed new light on an immuno-engineering solution for wound healing management in diabetic patients.
- Subjects :
- Scaffold
Population
Bioengineering
02 engineering and technology
Pharmacology
010402 general chemistry
01 natural sciences
Diabetes Mellitus, Experimental
Colloid and Surface Chemistry
Tissue engineering
medicine
Animals
Cytotoxic T cell
Physical and Theoretical Chemistry
education
Chemokine CCL2
Skin
Wound Healing
education.field_of_study
Tissue Scaffolds
integumentary system
business.industry
Macrophages
Monocyte
Surfaces and Interfaces
General Medicine
021001 nanoscience & nanotechnology
Streptozotocin
Immunohistochemistry
In vitro
0104 chemical sciences
Mice, Inbred C57BL
medicine.anatomical_structure
Immunology
Cattle
Female
0210 nano-technology
Wound healing
business
Biotechnology
medicine.drug
Subjects
Details
- ISSN :
- 09277765
- Volume :
- 145
- Database :
- OpenAIRE
- Journal :
- Colloids and Surfaces B: Biointerfaces
- Accession number :
- edsair.doi.dedup.....9acf4864a53dc7a36b7c52329e6d7eea