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A unified definition of clinical resistance/intolerance to hydroxyurea in essential thrombocythemia: results of a consensus process by an international working group
- Source :
- Barosi, G, Besses, C, Birgegard, G, Briere, J, Cervantes, F, Finazzi, G, Gisslinger, H, Griesshammer, M, Gugliotta, L, Harrison, C, Hasselbalch, H, Lengfelder, E, Reilly, J T, Michiels, J J & Barbui, T 2007, ' A unified definition of clinical resistance/intolerance to hydroxyurea in essential thrombocythemia : results of a consensus process by an international working group ', Leukemia, vol. 21, no. 2, pp. 277-80 . https://doi.org/10.1038/sj.leu.2404473, Leukemia
- Publication Year :
- 2006
- Publisher :
- Springer Science and Business Media LLC, 2006.
-
Abstract
- Udgivelsesdato: 2007-Feb BACKGROUND: Three main problems hamper the identification of wheat food allergens: (1) lack of a standardized procedure for extracting all of the wheat protein fractions; (2) absence of double-blind, placebo-controlled food challenge studies that compare the allergenic profile of Osborne's three protein fractions in subjects with real wheat allergy, and (3) lack of data on the differences in IgE-binding capacity between raw and cooked wheat. METHODS: Sera of 16 wheat-challenge-positive patients and 6 patients with wheat anaphylaxis, recruited from Italy, Denmark and Switzerland, were used for sodium dodecyl sulfate-polyacrylamide gel electrophoresis/immunoblotting of the three Osborne's protein fractions (albumin/globulin, gliadins and glutenins) of raw and cooked wheat. Thermal sensitivity of wheat lipid transfer protein (LTP) was investigated by spectroscopic approaches. IgE cross-reactivity between wheat and grass pollen was studied by blot inhibition. RESULTS: The most important wheat allergens were the alpha-amylase/trypsin inhibitor subunits, which were present in all three protein fractions of raw and cooked wheat. Other important allergens were a 9-kDa LTP in the albumin/globulin fraction and several low-molecular-weight (LMW) glutenin subunits in the gluten fraction. All these allergens showed heat resistance and lack of cross-reactivity to grass pollen allergens. LTP was a major allergen only in Italian patients. CONCLUSIONS: The alpha-amylase inhibitor was confirmed to be the most important wheat allergen in food allergy and to play a role in wheat-dependent exercise-induced anaphylaxis, too. Other important allergens were LTP and the LMW glutenin subunits.
- Subjects :
- Cancer Research
medicine.medical_specialty
Consensus Development Conferences as Topic
Drug Resistance
Antineoplastic Agents
Drug resistance
Body weight
Hydroxycarbamide
Internal medicine
otorhinolaryngologic diseases
medicine
Humans
Hydroxyurea
In patient
Thrombocythemia, Hemorrhagic
Essential thrombocythemia
business.industry
Patient Selection
food and beverages
Reproducibility of Results
Hematology
International working group
medicine.disease
Group discussion
Oncology
Human medicine
Hidroxicarbamida
business
Thrombocythemia, Essential
medicine.drug
Subjects
Details
- ISSN :
- 14765551 and 08876924
- Volume :
- 21
- Database :
- OpenAIRE
- Journal :
- Leukemia
- Accession number :
- edsair.doi.dedup.....9aa75ea71481ddf9fe4ad0d2c96cee7a