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Establishment of a quantitative and qualitative analysis and isolation method for tetracyclic iridoids from Morinda lucida Bentham leaves
- Source :
- Journal of Pharmaceutical and Biomedical Analysis. 164:475-480
- Publication Year :
- 2019
- Publisher :
- Elsevier BV, 2019.
-
Abstract
- A new high performance liquid chromatography (HPLC) method has been established for quantitative and qualitative analysis of three tetracyclic iridoids: ML-2-3 (1), molucidin (2), and ML-F52 (3), which are responsible for anti-trypanosomal and anti-leishmanial activities of Morinda lucida Bentham leaves. Separation of 1–3 from dried 80% aqueous (aq.) ethanol extract was achieved on a reversed-phase cholester column packed with cholesteryl-bonded silica using an acetonitrile-0.1% aq. formic acid mobile phase system. Ultraviolet-visible (UV-VIS) spectroscopy was employed for detection of compounds, and their contents were determined by measuring absorbance at 254 nm. Depending on the above system, several factors potentially affecting the concentration of tetracyclic iridoids were evaluated resulting in several variation on plant organs, seasonality, variation between individual trees, and branch positions within the trees. Moreover, we developed a simple, quick, and effective method for tetracyclic iridoid isolation from M. lucida leaves that consisted of extraction by sonication into 80% aq. ethanol, basic hydrolysis, acid neutralization, liquid-liquid extraction into an organic solvent, and reverse phase open column chromatography. Employing this method, we have succeeded to obtain 1 as a colorless crystal yielding of 0.23%, which was 28 times higher than that of previous isolation method. Setting up methodology in this paper may be important for future in vitro and in vivo studies of tetracyclic iridoids and moreover for their applications in new drug design and development.
- Subjects :
- Trypanosoma
Iridoid
Formic acid
medicine.drug_class
Clinical Biochemistry
Pharmaceutical Science
Chemical Fractionation
01 natural sciences
High-performance liquid chromatography
Analytical Chemistry
Absorbance
chemistry.chemical_compound
Column chromatography
Drug Discovery
medicine
Iridoids
Morinda
Chromatography, High Pressure Liquid
Qualitative Research
Spectroscopy
Aqueous solution
Chromatography
Plant Extracts
010405 organic chemistry
010401 analytical chemistry
Extraction (chemistry)
Trypanocidal Agents
0104 chemical sciences
Plant Leaves
chemistry
Drug Design
Solvents
Quantitative analysis (chemistry)
Subjects
Details
- ISSN :
- 07317085
- Volume :
- 164
- Database :
- OpenAIRE
- Journal :
- Journal of Pharmaceutical and Biomedical Analysis
- Accession number :
- edsair.doi.dedup.....9aa31e4e510d515e2757f71f301dd59d