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Non-thermal plasma specifically kills oral squamous cell carcinoma cells in a catalytic Fe(II)-dependent manner
- Source :
- Journal of Clinical Biochemistry and Nutrition
- Publication Year :
- 2019
- Publisher :
- the Society for Free Radical Research Japan, 2019.
-
Abstract
- Oral cancer accounts for ~2% of all cancers worldwide, and therapeutic intervention is closely associated with quality of life. Here, we evaluated the effects of non-thermal plasma on oral squamous cell carcinoma cells with special reference to catalytic Fe(II). Non-thermal plasma exerted a specific killing effect on oral squamous cell carcinoma cells in comparison to fibroblasts. Furthermore, the effect was dependent on the amounts of catalytic Fe(II), present especially in lysosomes. After non-thermal plasma application, lipid peroxidation occurred and peroxides and mitochondrial superoxide were generated. Cancer cell death by non-thermal plasma was promoted dose-dependently by prior application of ferric ammonium citrate and prevented by desferrioxamine, suggesting the association of ferroptosis. Potential involvement of apoptosis was also observed with positive terminal deoxynucleaotidyl transferase-mediated dUTP nick end labeling and annexin V results. Non-thermal plasma exposure significantly suppressed the migratory, invasive and colony-forming abilities of squamous cell carcinoma cells. The oral cavity is easily observable; therefore, non-thermal plasma can be directly applied to the oral cavity to kill oral squamous cell carcinoma without damaging fibroblasts. In conclusion, non-thermal plasma treatment is a potential therapeutic option for oral cancer.
- Subjects :
- 0301 basic medicine
non-thermal plasma
Clinical Biochemistry
Medicine (miscellaneous)
Nonthermal plasma
Catalysis
Lipid peroxidation
03 medical and health sciences
chemistry.chemical_compound
0302 clinical medicine
Annexin
medicine
catalytic ferrous iron
Basal cell
030109 nutrition & dietetics
Nutrition and Dietetics
apoptosis
Cancer
medicine.disease
ferroptosis
oral squamous cell carcinoma
stomatognathic diseases
chemistry
Apoptosis
Cancer cell
Cancer research
030211 gastroenterology & hepatology
Original Article
Subjects
Details
- Language :
- English
- ISSN :
- 18805086 and 09120009
- Volume :
- 65
- Issue :
- 1
- Database :
- OpenAIRE
- Journal :
- Journal of Clinical Biochemistry and Nutrition
- Accession number :
- edsair.doi.dedup.....9a9ae18596e20b02cae7bf96a494a097