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Assessment of bovine respiratory disease progression in calves challenged with bovine herpesvirus 1 and Mannheimia haemolytica using point-of-care and laboratory-based blood leukocyte differential assays
- Source :
- Translational Animal Science
- Publication Year :
- 2021
- Publisher :
- Oxford University Press (OUP), 2021.
-
Abstract
- Blood leukocyte differentials can be useful for understanding changes associated with bovine respiratory disease (BRD) progression. By improving turnaround time, point-of-care leukocyte differential assays (PCLD) may provide logistical advantages to laboratory-based assays. Our objective was to assess BRD progression in steers challenged with bovine herpesvirus 1 and Mannheimia haemolytica using point-of-care and laboratory-based blood leukocyte differentials. Thirty Holstein steers (average body weight of 211 kg + 2.4 kg) were inoculated intranasally on day 0 with bovine herpesvirus 1 and intrabronchially on day 6 with Mannheimia haemolytica. Blood leukocytes differentials were measured using both assays from study days 0 to 13. Linear mixed models were fitted to evaluate the associations between: (1) the type of assay (laboratory-based or PCLD) with respect to leukocyte, lymphocyte, and neutrophil concentrations; (2) study day with cell concentrations; and (3) cell concentrations with lung consolidation measured at necropsy. Point-of-care leukocyte, lymphocyte, and neutrophil concentrations were significantly associated (P < 0.05) with the respective cell concentrations obtained from the laboratory-based leukocyte differential. Cell concentrations reported by both assays differed significantly (P < 0.05) over time, indicating shifts from healthy to viral and bacterial disease states. Lymphocyte concentrations, lymphocyte/neutrophil ratios obtained from both assays, and band neutrophil concentrations from the laboratory-based assay were significantly associated (P < 0.05) with lung consolidation, enhancing assessments of disease severity. The PCLD may be a useful alternative to assess BRD progression when laboratory-based leukocyte differentials are impractical.
- Subjects :
- Bacterial disease
General Veterinary
biology
business.industry
of
Lymphocyte
Animal Health and Well Being
Cell
Bovine respiratory disease
MANNHEIMIA HAEMOLYTICA
point
care diagnostics
biology.organism_classification
medicine.disease
Bovine herpesvirus 1
Bovine Respiratory Disease Complex
medicine.anatomical_structure
Immunology
medicine
AcademicSubjects/SCI00960
Animal Science and Zoology
Nasal administration
bovine respiratory disease complex
leukocyte differentials
business
Subjects
Details
- ISSN :
- 25732102
- Volume :
- 5
- Database :
- OpenAIRE
- Journal :
- Translational Animal Science
- Accession number :
- edsair.doi.dedup.....9a97c85cdfcee5b3fda97798e1e41023