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Up-regulation of syntaxin1 in ischemic cortex after permanent focal ischemia in rats
- Source :
- Brain Research. 1272:52-61
- Publication Year :
- 2009
- Publisher :
- Elsevier BV, 2009.
-
Abstract
- Syntaxin1 and synaptotagmin are located in the pre-synaptic terminals and play central roles in Ca2+-triggered neurotransmitter release. Because excessive synaptic transmission has been implicated in neuronal cell death after ischemia, we investigated the effects of cerebral ischemia on the levels of these proteins using a rat permanent focal ischemia model. Western blot analysis revealed that the protein level of syntaxin1 was significantly up-regulated in the ischemic core cortex and peri-ischemic cortex at 1 day after ischemia, while the protein level of synaptotagmin was not. Immunohistochemical analysis revealed that the protein level of syntaxin1 was markedly up-regulated in the ischemic areas where immunoreaction for MAP2 was lost. Furthermore, we showed that resident microglial cells were quite vulnerable to ischemia. Our data provide novel insights into the molecular mechanism of cerebral ischemia at the pre-synaptic terminals.
- Subjects :
- Male
Pathology
medicine.medical_specialty
Central nervous system
Ischemia
Syntaxin 1
Neurotransmission
Synaptotagmin 1
Brain ischemia
Synaptotagmins
chemistry.chemical_compound
Rats, Inbred SHR
Cortex (anatomy)
Glial Fibrillary Acidic Protein
medicine
Animals
Neurotransmitter
Molecular Biology
Cerebral Cortex
Analysis of Variance
business.industry
General Neuroscience
Calcium-Binding Proteins
Microfilament Proteins
Infarction, Middle Cerebral Artery
medicine.disease
Rats
Up-Regulation
Disease Models, Animal
medicine.anatomical_structure
chemistry
Cerebral cortex
Neurology (clinical)
business
Microtubule-Associated Proteins
Neuroscience
Developmental Biology
Subjects
Details
- ISSN :
- 00068993
- Volume :
- 1272
- Database :
- OpenAIRE
- Journal :
- Brain Research
- Accession number :
- edsair.doi.dedup.....9a81b448c1e130a7b14b33040c7e183b
- Full Text :
- https://doi.org/10.1016/j.brainres.2009.03.047