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Up-regulation of syntaxin1 in ischemic cortex after permanent focal ischemia in rats

Authors :
Masahiro Sakanaka
Ryuji Hata
Fang Cao
Pengxiang Zhu
Michinobu Niinobe
Source :
Brain Research. 1272:52-61
Publication Year :
2009
Publisher :
Elsevier BV, 2009.

Abstract

Syntaxin1 and synaptotagmin are located in the pre-synaptic terminals and play central roles in Ca2+-triggered neurotransmitter release. Because excessive synaptic transmission has been implicated in neuronal cell death after ischemia, we investigated the effects of cerebral ischemia on the levels of these proteins using a rat permanent focal ischemia model. Western blot analysis revealed that the protein level of syntaxin1 was significantly up-regulated in the ischemic core cortex and peri-ischemic cortex at 1 day after ischemia, while the protein level of synaptotagmin was not. Immunohistochemical analysis revealed that the protein level of syntaxin1 was markedly up-regulated in the ischemic areas where immunoreaction for MAP2 was lost. Furthermore, we showed that resident microglial cells were quite vulnerable to ischemia. Our data provide novel insights into the molecular mechanism of cerebral ischemia at the pre-synaptic terminals.

Details

ISSN :
00068993
Volume :
1272
Database :
OpenAIRE
Journal :
Brain Research
Accession number :
edsair.doi.dedup.....9a81b448c1e130a7b14b33040c7e183b
Full Text :
https://doi.org/10.1016/j.brainres.2009.03.047