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Low glucocorticoid concentrations decrease oxidative phosphorylation of isolated rat brain mitochondria: an additional effect of dexamethasone
- Source :
- Fundamental and Clinical Pharmacology, Fundamental and Clinical Pharmacology, Wiley, 2000, 14 (5), pp.493-500. ⟨10.1111/j.1472-8206.2000.tb00432.x⟩, Fundamental & Clinical Pharmacology, Fundamental & Clinical Pharmacology, Wiley, 2000, 14 (5), pp.493-500. ⟨10.1111/j.1472-8206.2000.tb00432.x⟩
- Publication Year :
- 2000
- Publisher :
- HAL CCSD, 2000.
-
Abstract
- The effects of hydrocortisone, triamcinolone, prednisolone and dexamethasone have been investigated in vitro using mitochondria isolated from rat brain. Respiratory control ratio (RCR), oxygen consumption, ATP synthesis, enzymatic activities of involved complexes and superoxide anion generation have been measured to assess the effects of these drugs. Our data showed that the decrease of RCR induced by glucocorticoids was due to a common inhibition of oxidative phosphorylation (State 3) and of complex V activity and a modification of the proton-fluxes through the mitochondrial inner membrane. These effects were quantitatively limited, since they occurred at concentrations lower than 2 nM. Dexamethasone was the only one able to induce a specific inhibition of complex I activity and to decrease the superoxide anion radical generation. Inhibition of complex V and partial reversion of uncoupling seem to be common properties of glucocorticoids. The theoretical consequence of these inhibitions could be the modulation of the mitochondrial function, oxygen consumption rate, ATP synthase activity and superoxide anion radical production, involved in many patho-physiological phenomena.
- Subjects :
- Male
medicine.medical_specialty
Hydrocortisone
Prednisolone
[SDV.NEU.NB]Life Sciences [q-bio]/Neurons and Cognition [q-bio.NC]/Neurobiology
Respiratory chain
Oxidative phosphorylation
[SDV.BC.BC]Life Sciences [q-bio]/Cellular Biology/Subcellular Processes [q-bio.SC]
In Vitro Techniques
Mitochondrion
Biology
Triamcinolone
Dexamethasone
Electron Transport Complex III
03 medical and health sciences
chemistry.chemical_compound
Oxygen Consumption
0302 clinical medicine
[SDV.SP.MED]Life Sciences [q-bio]/Pharmaceutical sciences/Medication
Internal medicine
[SDV.BC.IC]Life Sciences [q-bio]/Cellular Biology/Cell Behavior [q-bio.CB]
medicine
Animals
Pharmacology (medical)
Phosphorylation
Rats, Wistar
[SDV.BBM.BC]Life Sciences [q-bio]/Biochemistry, Molecular Biology/Biochemistry [q-bio.BM]
Inner mitochondrial membrane
Glucocorticoids
ComputingMilieux_MISCELLANEOUS
030304 developmental biology
Pharmacology
0303 health sciences
ATP synthase
[SDV.BBM.BS]Life Sciences [q-bio]/Biochemistry, Molecular Biology/Structural Biology [q-bio.BM]
Superoxide
Brain
Mitochondria
Rats
3. Good health
Endocrinology
chemistry
Mechanism of action
biology.protein
[SDV.SP.PHARMA]Life Sciences [q-bio]/Pharmaceutical sciences/Pharmacology
medicine.symptom
Reactive Oxygen Species
030217 neurology & neurosurgery
Subjects
Details
- Language :
- English
- ISSN :
- 07673981 and 14728206
- Database :
- OpenAIRE
- Journal :
- Fundamental and Clinical Pharmacology, Fundamental and Clinical Pharmacology, Wiley, 2000, 14 (5), pp.493-500. ⟨10.1111/j.1472-8206.2000.tb00432.x⟩, Fundamental & Clinical Pharmacology, Fundamental & Clinical Pharmacology, Wiley, 2000, 14 (5), pp.493-500. ⟨10.1111/j.1472-8206.2000.tb00432.x⟩
- Accession number :
- edsair.doi.dedup.....9a68871afb765c798994069f54f7d874