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Transcriptional dynamics of transposable elements when converting fibroblast cells of Macaca mulatta to neuroepithelial stem cells
- Source :
- BMC Genomics, BMC Genomics, Vol 22, Iss S3, Pp 1-13 (2021)
- Publication Year :
- 2021
- Publisher :
- BioMed Central, 2021.
-
Abstract
- Background Transposable elements (TE) account for more than 50% of human genome. It has been reported that some types of TEs are dynamically regulated in the reprogramming of human cell lines. However, it is largely unknown whether some TEs in Macaca mulatta are also regulated during the reprogramming of cell lines of monkey. Results Here, we systematically examined the transcriptional activities of TEs during the conversion of Macaca mulatta fibroblast cells to neuroepithelial stem cells (NESCs). Hundreds of TEs were dynamically regulated during the reprogramming of Macaca mulatta fibroblast cells. Furthermore, 48 Long Terminal Repeats (LTRs), as well as some integrase elements, of Macaca endogenous retrovirus 3 (MacERV3) were transiently activated during the early stages of the conversion process, some of which were further confirmed with PCR experiments. These LTRs were potentially bound by critical transcription factors for reprogramming, such as KLF4 and ETV5. Conclusion These results suggest that the transcription of TEs are delicately regulated during the reprogramming of Macaca mulatta fibroblast cells. Although the family of ERVs activated during the reprogramming of fibroblast cells in Macaca mulatta is different from those in the reprogramming of human fibroblast cells, our results suggest that the activation of some ERVs is a conserved mechanism in primates for converting fibroblast cells to stem cells.
- Subjects :
- animal diseases
Endogenous retrovirus
QH426-470
Biology
03 medical and health sciences
Kruppel-Like Factor 4
0302 clinical medicine
stomatognathic system
Genetics
medicine
Long terminal repeat (LTR)
Animals
Humans
Fibroblast
030304 developmental biology
0303 health sciences
Research
Neuroepithelial stem cell (NESC)
Stem Cells
Terminal Repeat Sequences
food and beverages
Fibroblasts
Macaca mulatta
Long terminal repeat
Cell biology
Neuroepithelial cell
Induced pluripotent stem cell (iPSC)
medicine.anatomical_structure
KLF4
Cell culture
DNA Transposable Elements
Transposable element (TE)
Stem cell
Reprogramming
Transcription
TP248.13-248.65
030217 neurology & neurosurgery
Endogenous retrovirus (ERV)
Biotechnology
Subjects
Details
- Language :
- English
- ISSN :
- 14712164
- Volume :
- 22
- Issue :
- Suppl 3
- Database :
- OpenAIRE
- Journal :
- BMC Genomics
- Accession number :
- edsair.doi.dedup.....9a61548676a6f90a09712d559c389499