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Examining the effect of wound cleansing on the microbiome of venous stasis ulcers
- Source :
- Wound Repair and Regeneration. 29:766-776
- Publication Year :
- 2021
- Publisher :
- Wiley, 2021.
-
Abstract
- Common treatment for venous leg wounds includes topical wound dressings with compression. At each dressing change, wounds are debrided and washed; however, the effect of the washing procedure on the wound microbiome has not been studied. We hypothesized that wound washing may alter the wound microbiome. To characterize microbiome changes with respect to wound washing, swabs from 11 patients with chronic wounds were sampled before and after washing, and patient microbiomes were characterized using 16S rRNA sequencing and culturing. Microbiomes across patient samples prior to washing were typically polymicrobial but varied in the number and type of bacterial genera present. Proteus and Pseudomonas were the dominant genera in the study. We found that washing does not consistently change microbiome diversity but does cause consistent changes in microbiome composition. Specifically, washing caused a decrease in the relative abundance of the most highly represented genera in each patient cluster. The finding that venous leg ulcer wound washing, a standard of care therapy, can induce changes in the wound microbiome is novel and could be potentially informative for future guided therapy strategies.
- Subjects :
- Wound Healing
Standard of care
integumentary system
business.industry
Microbiota
Venous Stasis Ulcers
Dermatology
medicine.disease
Bandages
Venous leg ulcer
Dressing change
Varicose Ulcer
Microbiology
030207 dermatology & venereal diseases
03 medical and health sciences
0302 clinical medicine
RNA, Ribosomal, 16S
medicine
Humans
Surgery
Microbiome
Wound cleansing
business
Wound treatment
Subjects
Details
- ISSN :
- 1524475X and 10671927
- Volume :
- 29
- Database :
- OpenAIRE
- Journal :
- Wound Repair and Regeneration
- Accession number :
- edsair.doi.dedup.....9a5add7af45d347d9dfd5a3e37083286