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Bioimaging assessment and effect of skin wound healing using bone-marrow-derived mesenchymal stromal cells with the artificial dermis in diabetic rats
- Source :
- Journal of Biomedical Optics. 13:064036
- Publication Year :
- 2008
- Publisher :
- SPIE-Intl Soc Optical Eng, 2008.
-
Abstract
- We investigate the relationship between the fate and healing effect of transplanted mesenchymal stromal cells (MSCs) in a rat diabetic skin wound model. Rats are treated with streptozotocin to induce diabetic conditions. A full-thickness skin defect is surgically made on the head of diabetic rats, and covered with an artificial dermis impregnated with either bone marrow cells (BMCs) or bone-marrow-derived MSCs from firefly luciferase (luc) transgenic (Tg) rats. Wound healing is evaluated using planimetry and immunohistochemistry, and the fate of transplanted MSCs is determined using in-vivo luminescent imaging. The diabetic wound treated with MSCs-impregnated artificial dermis is significantly smaller than that treated with artificial dermis alone at 1 week postoperation. Photons of luc+ MSCs are detected at the transplanted site during healing (3 weeks), whereas those of luc+ MSCs are depleted only after 1 week postimplantation. Immunohistochemistry at the healing site treated with MSCs demonstrates that CD31+ vessels increase with expression of vascular endothelial growth factor, suggesting that MSCs accelerate angiogenesis. These findings suggest that transplanted MSCs could be retained at wound sites during the healing process in a diabetic rat model, and subsequently promote wound healing through angiogenesis.
- Subjects :
- Male
CD31
medicine.medical_specialty
Pathology
Angiogenesis
Biomedical Engineering
Mesenchymal Stem Cell Transplantation
Diabetes Complications
Biomaterials
chemistry.chemical_compound
Dermis
Skin Ulcer
medicine
Animals
Bone Marrow Transplantation
Skin, Artificial
Wound Healing
integumentary system
business.industry
Mesenchymal stem cell
Mesenchymal Stem Cells
Atomic and Molecular Physics, and Optics
Rats
Electronic, Optical and Magnetic Materials
Surgery
Transplantation
Vascular endothelial growth factor
Disease Models, Animal
Treatment Outcome
medicine.anatomical_structure
Microscopy, Fluorescence
chemistry
Rats, Inbred Lew
Luminescent Measurements
Bone marrow
business
Wound healing
Subjects
Details
- ISSN :
- 10833668
- Volume :
- 13
- Database :
- OpenAIRE
- Journal :
- Journal of Biomedical Optics
- Accession number :
- edsair.doi.dedup.....9a38b66eaeb8efeaf1dc4c7cd70b1d08