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A novel dynein-type AAA+ protein with peroxisomal targeting signal type 2
- Source :
- The Journal of Biochemistry. 167:429-432
- Publication Year :
- 2020
- Publisher :
- Oxford University Press (OUP), 2020.
-
Abstract
- Peroxisomal matrix proteins are imported into peroxisomes in a process mediated by peroxisomal targeting signal (PTS) type 1 and 2. The PTS2 proteins are imported into peroxisomes after binding with Pex7p. Niwa et al. (A newly isolated Pex7-binding, atypical PTS2 protein P7BP2 is a novel dynein-type AAA+ protein. J Biochem 2018;164:437–447) identified a novel Pex7p-binding protein in CHO cells and characterized the subcellular distribution and molecular properties of the human homologue, ‘P7BP2’. Interestingly, P7BP2 possesses PTS2 at the NH2 terminal and six putative AAA+ domains. Another group has suggested that the protein also possesses mitochondrial targeting signal at the NH2 terminal. In fact, the P7BP2 expressed in mammalian cells is targeted to both peroxisomes and mitochondria. The purified protein from Sf9 cells is a monomer and has a disc-like ring structure, suggesting that P7BP2 is a novel dynein-type AAA+ family protein. The protein expressed in insect cells exhibits ATPase activity. P7BP2 localizes to peroxisomes and mitochondria, and has a common function related to dynein-type ATPases in both organelles.
- Subjects :
- Adenosine Triphosphatases
0303 health sciences
Chemistry
Peroxisomal matrix
Chinese hamster ovary cell
030302 biochemistry & molecular biology
Dynein
Dyneins
Sf9
macromolecular substances
General Medicine
Peroxisomal Targeting Signal 2 Receptor
Peroxisome
Biochemistry
Peroxisomal Targeting Signals
Cell biology
03 medical and health sciences
Dynein ATPase
Peroxisomes
Humans
Molecular Biology
Peroxisomal targeting signal
030304 developmental biology
Subjects
Details
- ISSN :
- 17562651 and 0021924X
- Volume :
- 167
- Database :
- OpenAIRE
- Journal :
- The Journal of Biochemistry
- Accession number :
- edsair.doi.dedup.....9a387421783ba7c7dcea50a8c37ab4bf