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Resolution extension by image summing in serial femtosecond crystallography of two-dimensional membrane-protein crystals
- Source :
- IUCrJ, Vol 5, Iss 1, Pp 103-117 (2018), IUCrJ, IUCrJ 5(1), 103-117 (2018). doi:10.1107/S2052252517017043, IUCrJ, vol 5, iss Pt 1
- Publication Year :
- 2018
- Publisher :
- International Union of Crystallography (IUCr), 2018.
-
Abstract
- IUCrJ 5(1), 103 - 117 (2018). doi:10.1107/S2052252517017043<br />Previous proof-of-concept measurements on single-layer two-dimensional membrane-protein crystals performed at X-ray free-electron lasers (FELs) have demonstrated that the collection of meaningful diffraction patterns, which is not possible at synchrotrons because of radiation-damage issues, is feasible. Here, the results obtained from the analysis of a thousand single-shot, room-temperature X-ray FEL diffraction images from two-dimensional crystals of a bacteriorhodopsin mutant are reported in detail. The high redundancy in the measurements boosts the intensity signal-to-noise ratio, so that the values of the diffracted intensities can be reliably determined down to the detector-edge resolution of 4 Å. The results show that two-dimensional serial crystallography at X-ray FELs is a suitable method to study membrane proteins to near-atomic length scales at ambient temperature. The method presented here can be extended to pump–probe studies of optically triggered structural changes on submillisecond timescales in two-dimensional crystals, which allow functionally relevant large-scale motions that may be quenched in three-dimensional crystals.<br />Published by IUCr, Chester
- Subjects :
- 0301 basic medicine
Diffraction
Materials science
Physics::Optics
Bioengineering
membrane proteins
Atomic
Biochemistry
law.invention
03 medical and health sciences
Particle and Plasma Physics
law
Nuclear
ddc:530
serial crystallography
General Materials Science
two-dimensional crystals
Crystallography
biology
Resolution (electron density)
Molecular
Bacteriorhodopsin
General Chemistry
Condensed Matter Physics
Laser
Research Papers
030104 developmental biology
QD901-999
Femtosecond
biology.protein
free-electron lasers
Physical Chemistry (incl. Structural)
Subjects
Details
- ISSN :
- 20522525
- Volume :
- 5
- Database :
- OpenAIRE
- Journal :
- IUCrJ
- Accession number :
- edsair.doi.dedup.....9a34cdc396a6cf12e1343b89e8f0d092