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Measurement of Angiotensin Peptides: HPLC-RIA
- Source :
- Hypertension ISBN: 9781493966233
- Publication Year :
- 2017
- Publisher :
- Springer New York, 2017.
-
Abstract
- The renin-angiotensin system (RAS) is a complex circulating and tissue-based system. There are multiple pathways for the formation and degradation of peptides. In order to understand the functions of the system, characterization of angiotensin peptides (products and substrates) is important. Radioimmunoassays with the requisite specificity and sensitivity have been developed to allow for the characterization and quantification of circulating and tissue angiotensins. Here, we describe the appropriate methods for collecting the tissue and blood, the extractions steps required to partially purify and remove larger molecular weight-interfering proteins from tissue and plasma, and the radioimmunoassay of three of the peptides of this system (Ang I, Ang II, and Ang-(1-7)), as well as the verification of immunoreactive identity for Ang II and Ang-(1-7) by combined high-performance liquid chromatography-RIA analysis.
- Subjects :
- 0301 basic medicine
Chromatography
Chemistry
Angiotensin II
Radioimmunoassay
030204 cardiovascular system & hematology
High-performance liquid chromatography
Article
Peptide Fragments
Renin-Angiotensin System
03 medical and health sciences
030104 developmental biology
0302 clinical medicine
Renin–angiotensin system
Animals
Humans
Angiotensin I
Hplc ria
Chromatography, High Pressure Liquid
hormones, hormone substitutes, and hormone antagonists
Homogenization (biology)
Subjects
Details
- ISBN :
- 978-1-4939-6623-3
- ISBNs :
- 9781493966233
- Database :
- OpenAIRE
- Journal :
- Hypertension ISBN: 9781493966233
- Accession number :
- edsair.doi.dedup.....9a12b59445df789a88bb8689c4a52ad0