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Different Approaches to Unveil Biomolecule Configurations and Their Mutual Interactions
- Source :
- Analytical Letters
- Publication Year :
- 2021
-
Abstract
- A novel technique was demonstrated that overcome important drawbacks to crosslink cells by irradiation with ultrashort UV laser pulses (L-crosslinking). To use this technique coupled to Chromatin ImmunoPrecipitation (ChIP) in a high throughput context, a pre-screening fast method needs to be implemented to set up suitable irradiation conditions of the cell sample for efficient L-crosslinking with no final and long ChIP analysis. Here a fast method is reported where living human cells have been first transfected with a vector coding for Estrogen Receptor {\alpha} (ER{\alpha}), linked to Green Florescent protein (ER{\alpha}-GFP), so that the well-known interaction between the Estrogen Receptor Elements (ERE) region of the cell DNA and the ER{\alpha} protein can be detected by studying the fluorometric response of the irradiated cells. The damage induced to cells by UV irradiation is characterized by looking at DNA integrity, proteins stability and cellular viability. A second novel approach is presented to analyze or re-visit DNA and RNA sequences and their molecular configurations. This approach is based on methods derived from Chern-Simons super-gravity adapted to describe mutations in DNA/RNA strings, as well as interactions between nucleic acids. As a preliminary case we analyze the KRAS human gene sequence and some of its mutations. Interestingly, our model shows how the Chern-Simons current are capable to characterize the mutations within a sequence, in particular giving a quantitative indication of the mutation likelihood.<br />Comment: 33 pages, 5 figures
- Subjects :
- Novel technique
Chern-Simons current
Clinical Biochemistry
optical physics
Nanotechnology
02 engineering and technology
medicine.disease_cause
01 natural sciences
Biochemistry
Analytical Chemistry
law.invention
law
Electrochemistry
medicine
Chromatin analysi
Irradiation
Molecular Biology
Spectroscopy
chemistry.chemical_classification
Chemistry
Biomolecule
010401 analytical chemistry
Biochemistry (medical)
Molecular biophysics
Optical physics
Biomolecules (q-bio.BM)
021001 nanoscience & nanotechnology
Laser
0104 chemical sciences
genetic code
Quantitative Biology - Biomolecules
molecular biophysic
FOS: Biological sciences
0210 nano-technology
Ultraviolet
Subjects
Details
- Language :
- English
- ISSN :
- 00032719
- Database :
- OpenAIRE
- Journal :
- Analytical Letters
- Accession number :
- edsair.doi.dedup.....9a0f354faa8fbaedc31221122e205ff4