Back to Search
Start Over
Cardio-protection of salvianolic acid B through inhibition of apoptosis network
- Source :
- PLoS ONE, Vol 6, Iss 9, p e24036 (2011), PLoS ONE
- Publication Year :
- 2011
- Publisher :
- Public Library of Science (PLoS), 2011.
-
Abstract
- Targeting cellular function as a system rather than on the level of the single target significantly increases therapeutic potency. In the present study, we detect the target pathway of salvianolic acid B (SalB) in vivo. Acute myocardial infarction (AMI) was induced in rats followed by the treatment with 10 mg/kg SalB. Hemodynamic detection and pathological stain, 2-dimensional electrophoresis, MALDI-TOF MS/MS, Western blot, pathway identification, apoptosis assay and transmission electron microscope were used to elucidate the effects and mechanism of SalB on cardioprotection. Higher SalB concentration was found in ischemic area compared to no-ischemic area of heart, correlating with improved heart function and histological structure. Thirty-three proteins regulated by SalB in AMI rats were identified by biochemical analysis and were classified as the components of metabolism and apoptosis networks. SalB protected cardiomyocytes from apoptosis, inhibited poly (ADP-ribose) polymerase-1 pathway, and improved the integrity of mitochondrial and nucleus of heart tissue during AMI. Furthermore, the protective effects of SalB against apoptosis were verified in H9c2 cells. Our results provide evidence that SalB regulates multi-targets involved in the apoptosis pathway during AMI and therefore may be a candidate for novel therapeutics of heart diseases.
- Subjects :
- Proteomics
Male
Anatomy and Physiology
Critical Care and Emergency Medicine
Myocardial Infarction
Poly (ADP-Ribose) Polymerase-1
lcsh:Medicine
Apoptosis
Mitochondrion
Pharmacology
Cardiovascular
Cardiovascular System
Biochemistry
Tandem Mass Spectrometry
Molecular Cell Biology
Signaling in Cellular Processes
Electrophoresis, Gel, Two-Dimensional
Myocytes, Cardiac
lcsh:Science
Energy-Producing Organelles
Apoptotic Signaling Cascade
Apoptotic Signaling
Cardioprotection
Multidisciplinary
medicine.diagnostic_test
Cell Death
Chemistry
NF-kappa B
Cellular Structures
Signaling Cascades
Mitochondria
Medicine
medicine.symptom
Signal transduction
Poly(ADP-ribose) Polymerases
Research Article
Signal Transduction
Poly ADP ribose polymerase
Blotting, Western
Bioenergetics
Cardiovascular Pharmacology
Cell Line
Western blot
Microscopy, Electron, Transmission
In vivo
medicine
Cardiovascular Diseases in Women
In Situ Nick-End Labeling
Animals
Rats, Wistar
Biology
Benzofurans
Cell Nucleus
Acute Cardiovascular Problems
Myocardium
lcsh:R
Hemodynamics
Hypoxia (medical)
Molecular biology
Rats
Subcellular Organelles
Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
Cardiovascular Anatomy
lcsh:Q
Subjects
Details
- Language :
- English
- ISSN :
- 19326203
- Volume :
- 6
- Issue :
- 9
- Database :
- OpenAIRE
- Journal :
- PLoS ONE
- Accession number :
- edsair.doi.dedup.....9a02871f95321644001ad6899f2dc11f