Back to Search
Start Over
Graph theoretic network analysis reveals protein pathways underlying cell death following neurotropic viral infection
- Source :
- Scientific Reports
- Publication Year :
- 2015
- Publisher :
- Nature Publishing Group, 2015.
-
Abstract
- Complex protein networks underlie any cellular function. Certain proteins play a pivotal role in many network configurations, disruption of whose expression proves fatal to the cell. An efficient method to tease out such key proteins in a network is still unavailable. Here, we used graph-theoretic measures on protein-protein interaction data (interactome) to extract biophysically relevant information about individual protein regulation and network properties such as formation of function specific modules (sub-networks) of proteins. We took 5 major proteins that are involved in neuronal apoptosis post Chandipura Virus (CHPV) infection as seed proteins in a database to create a meta-network of immediately interacting proteins (1st order network). Graph theoretic measures were employed to rank the proteins in terms of their connectivity and the degree upto which they can be organized into smaller modules (hubs). We repeated the analysis on 2nd order interactome that includes proteins connected directly with proteins of 1st order. FADD and Casp-3 were connected maximally to other proteins in both analyses, thus indicating their importance in neuronal apoptosis. Thus, our analysis provides a blueprint for the detection and validation of protein networks disrupted by viral infections.
- Subjects :
- Programmed cell death
Cell
Computational biology
Biology
Bioinformatics
Interactome
Article
Mice
Rhabdoviridae Infections
Protein Interaction Mapping
medicine
Animals
Rank (graph theory)
Protein Interaction Maps
FADD
Neurons
Multidisciplinary
Cell Death
Computational Biology
Vesiculovirus
medicine.anatomical_structure
Order (biology)
biology.protein
Signal transduction
Function (biology)
Signal Transduction
Subjects
Details
- Language :
- English
- ISSN :
- 20452322
- Database :
- OpenAIRE
- Journal :
- Scientific Reports
- Accession number :
- edsair.doi.dedup.....99e9ecb735aa11885102cbe2848df732
- Full Text :
- https://doi.org/10.1038/srep14438