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Large-Scale Plasma Analysis Revealed New Mechanisms and Molecules Associated with the Host Response to SARS-CoV-2
- Source :
- International Journal of Molecular Sciences, Vol 21, Iss 8623, p 8623 (2020), International Journal of Molecular Sciences, Volume 21, Issue 22
- Publication Year :
- 2020
- Publisher :
- MDPI AG, 2020.
-
Abstract
- The novel severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has spread to nearly every continent, registering over 1,250,000 deaths worldwide. The effects of SARS-CoV-2 on host targets remains largely limited, hampering our understanding of Coronavirus Disease 2019 (COVID-19) pathogenesis and the development of therapeutic strategies. The present study used a comprehensive untargeted metabolomic and lipidomic approach to capture the host response to SARS-CoV-2 infection. We found that several circulating lipids acted as potential biomarkers, such as phosphatidylcholine 14:0_22:6 (area under the curve (AUC) = 0.96), phosphatidylcholine 16:1_22:6 (AUC = 0.97), and phosphatidylethanolamine 18:1_20:4 (AUC = 0.94). Furthermore, triglycerides and free fatty acids, especially arachidonic acid (AUC = 0.99) and oleic acid (AUC = 0.98), were well correlated to the severity of the disease. An untargeted analysis of non-critical COVID-19 patients identified a strong alteration of lipids and a perturbation of phenylalanine, tyrosine and tryptophan biosynthesis, phenylalanine metabolism, aminoacyl-tRNA degradation, arachidonic acid metabolism, and the tricarboxylic acid (TCA) cycle. The severity of the disease was characterized by the activation of gluconeogenesis and the metabolism of porphyrins, which play a crucial role in the progress of the infection. In addition, our study provided further evidence for considering phospholipase A2 (PLA2) activity as a potential key factor in the pathogenesis of COVID-19 and a possible therapeutic target. To date, the present study provides the largest untargeted metabolomics and lipidomics analysis of plasma from COVID-19 patients and control groups, identifying new mechanisms associated with the host response to COVID-19, potential plasma biomarkers, and therapeutic targets.
- Subjects :
- Male
Pharmacology
Pathogenesis
lcsh:Chemistry
chemistry.chemical_compound
Amino Acids
Tyrosine
lcsh:QH301-705.5
Spectroscopy
Aged, 80 and over
Arachidonic Acid
biology
Chemistry
Area under the curve
General Medicine
Middle Aged
Computer Science Applications
Metabolome
Phosphatidylcholines
Female
Arachidonic acid
Coronavirus Infections
Citric Acid Cycle
Pneumonia, Viral
fatty acids
Article
Catalysis
Inorganic Chemistry
Phospholipase A2
Lipidomics
Humans
Physical and Theoretical Chemistry
Pandemics
Molecular Biology
Triglycerides
Aged
SARS-CoV-2
Phosphatidylethanolamines
Organic Chemistry
Gluconeogenesis
COVID-19
biomarkers
Metabolism
Phospholipases A2
lcsh:Biology (General)
lcsh:QD1-999
biology.protein
metabolism
Oleic Acid
Subjects
Details
- Language :
- English
- ISSN :
- 16616596 and 14220067
- Volume :
- 21
- Issue :
- 8623
- Database :
- OpenAIRE
- Journal :
- International Journal of Molecular Sciences
- Accession number :
- edsair.doi.dedup.....99e1bd1a31ef648906323566eee8577c