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HBO Alleviates Neural Stem Cell Pyroptosis via lncRNA-H19/miR-423-5p/NLRP3 Axis and Improves Neurogenesis after Oxygen Glucose Deprivation
- Source :
- Oxidative medicine and cellular longevity. 2022
- Publication Year :
- 2021
-
Abstract
- Due to the limited neurogenesis capacity, there has been a big challenge in better recovery from neurological dysfunction caused by stroke for a long time. Neural stem cell (NSC) programmed death is one of the unfavorable factors for neural regeneration after stroke. The types of death such as apoptosis and necroptosis have been deeply investigated while the pyroptosis of NSCs is not quite understood. Although it is well accepted that hyperbaric oxygen (HBO) alleviates the oxygen-glucose deprivation (OGD) injury after stroke and reduces programmed death of NSCs, whether NSC pyroptosis is involved in this process is still unknown. Therefore, this study is aimed at studying the potential effect of HBO treatment on NSC pyroptosis following OGD exposure, as well as its influence on NSC proliferation and differentiation in vitro. The results revealed that OGD increased NOD-like receptor protein 3 (NLRP3) expression to induce the pyroptotic death of NSCs, which was rescued by HBO treatment. And the upregulated lncRNA-H19 functioned as a molecular sponge of miR-423-5p to target NLRP3 for NSC pyroptosis following OGD. Most importantly, it was confirmed that HBO exerted protection of NSCs against pyroptosis by inhibiting lncRNA-H19/miR-423-5p/NLRP3 axis. Moreover, HBO restraint of lncRNA-H19-associated pyroptosis benefited the proliferation and neuronal differentiation of NSCs. It was concluded that HBO attenuated NSC pyroptosis via lncRNA-H19/miR-423-5p/NLRP3 axis and enhanced neurogenesis following OGD. The findings provide new insight into NSC programmed death and enlighten therapeutic strategy after stroke.
- Subjects :
- Aging
Article Subject
Neurogenesis
Cell Differentiation
Cell Biology
General Medicine
Transfection
Biochemistry
nervous system diseases
Oxygen
MicroRNAs
Glucose
nervous system
Neural Stem Cells
Pyroptosis
Animals
Humans
RNA, Long Noncoding
biological phenomena, cell phenomena, and immunity
reproductive and urinary physiology
Subjects
Details
- ISSN :
- 19420994
- Volume :
- 2022
- Database :
- OpenAIRE
- Journal :
- Oxidative medicine and cellular longevity
- Accession number :
- edsair.doi.dedup.....99d44002aa7520f443ac9a2ae6794c74