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Development of a Rat Model of Sick Sinus Syndrome Using Pinpoint Press Permeation
- Source :
- BioMed Research International, Vol 2018 (2018), BioMed Research International
- Publication Year :
- 2018
- Publisher :
- Hindawi Limited, 2018.
-
Abstract
- Objective. Sick sinus syndrome (SSS) is one of the most common causes of cardiac impairment necessitating pacemaker implantation. However, studies of SSS pathogenesis are neither comprehensive nor conclusive due to limited success in achieving a stable rat SSS model. Here, we modified pinpoint press permeation to establish a stable rat SSS model. Methods. We randomly assigned 138 male Sprague-Dawley rats into three groups: normal control (n = 8), sham (n = 10), and SSS (n = 120). Postoperatively, the SSS group was further divided into SSSA (n = 40), SSSB (n = 40), and SSSC (n = 40), based on reduction in heart rates by 20–30%, 31–40%, and 41–50%, respectively. We also assessed histomorphological characteristics and hyperpolarization-activated cyclic nucleotide-gated cation channel 4 (HCN4) expression in the sinoatrial node (SAN) at 1, 2, 3, and 4 weeks after surgery. Results. Mortality was statistically higher in SSSC compared to SSSA and SSSB (7.5% versus 90.0% and 87.5%; P < 0.05). Heart rate in SSSA was gradually restored to preoperative levels by week 4 after surgery. In contrast, heart rate in SSSB was stable at 2–3 weeks after surgery. However, we observed that the tissues and cells in SAN were severely injured and also found a time-dependent increase in collagen content and atrium myocardium in SSSB. HCN4 expression was significantly reduced at all 4 time points in SSSB, with statistically significant differences among the groups (P < 0.01). Conclusion. We successfully developed a rat SSS model that was sustainable for up to 4 weeks.
- Subjects :
- Male
0301 basic medicine
Article Subject
Rat model
lcsh:Medicine
030204 cardiovascular system & hematology
General Biochemistry, Genetics and Molecular Biology
Sick sinus syndrome
Rats, Sprague-Dawley
Pathogenesis
03 medical and health sciences
0302 clinical medicine
Heart Rate
Heart rate
Hyperpolarization-Activated Cyclic Nucleotide-Gated Channels
medicine
Animals
Heart Atria
Normal control
Sinoatrial Node
Sick Sinus Syndrome
General Immunology and Microbiology
Atrium (architecture)
Sinoatrial node
business.industry
lcsh:R
General Medicine
medicine.disease
Rats
SSS
Disease Models, Animal
030104 developmental biology
medicine.anatomical_structure
Anesthesia
business
Research Article
Subjects
Details
- Language :
- English
- ISSN :
- 23146141 and 23146133
- Volume :
- 2018
- Database :
- OpenAIRE
- Journal :
- BioMed Research International
- Accession number :
- edsair.doi.dedup.....99c8e7e8d7ab0e964c889dabec22e24f