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Impact of Sequence Specificity of Spherical Nucleic Acids on Macrophage Activation in Vitro and in Vivo
- Publication Year :
- 2019
-
Abstract
- The effects of spherical nucleic acid (SNA) gold nanoparticle conjugates on the activation of macrophages in vitro and release of cytokines in vivo were explored. Herein, we show that G-quadruplexes, the formation of which is enhanced on gold nanoparticle surfaces, elicit an increase in cytokine release from mouse and human macrophages and induce the upregulation of activation receptors as well as NO(2) production in vitro. Moreover, these G-rich SNAs can induce cytokine release when injected intravenously, though there were no severe, long-term effects observed. These results further reinforce the notion that nucleic acid sequence and structure play an important role in how SNAs interact in biological milieu and highlight a key design parameter.
- Subjects :
- medicine.medical_treatment
Nitrogen Dioxide
Pharmaceutical Science
Metal Nanoparticles
02 engineering and technology
In Vitro Techniques
030226 pharmacology & pharmacy
Article
03 medical and health sciences
Mice
0302 clinical medicine
Downregulation and upregulation
In vivo
Nucleic Acids
Drug Discovery
medicine
Macrophage
Animals
Humans
Receptor
Cells, Cultured
Chemistry
Macrophages
Macrophage Activation
021001 nanoscience & nanotechnology
In vitro
Cell biology
G-Quadruplexes
Mice, Inbred C57BL
Cytokine
Spherical nucleic acid
Nucleic acid
Molecular Medicine
Cytokines
Gold
0210 nano-technology
Subjects
Details
- Language :
- English
- Database :
- OpenAIRE
- Accession number :
- edsair.doi.dedup.....99a1c4fac76b00ae24ec6a56705bc9c5