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Evaluation of PLGA microsphere size effect on myotoxicity using the isolated rodent skeletal muscle model
- Source :
- Pharmaceutical development and technology. 1(3)
- Publication Year :
- 1996
-
Abstract
- The present work investigated the magnitude of microsphere-induced acute myotoxicity and determined whether this myotoxicity is related to microsphere size and/or reconstitution solvent. Using a high molecular weight poly(dl-lactide-co-glycolide) copolymer, the myotoxicity of two different size microsphere formulations (3.6 microns and 19 microns) in normal saline or distilled water was quantified using a previously validated isolated rat muscle system. Overall, microspheres were found to be relatively nontoxic compared to known myotoxic agents (e.g., phenytoin) and control muscles. The smaller microspheres were found to be significantly more myotoxic than larger microspheres. Furthermore, the myotoxicity was lower in large microspheres reconstituted with normal saline or normal saline with 0.5% (w/v) carboxymethylcellulose (to prevent aggregation) compared to those reconstituted with distilled water. Smaller microspheres were found to be extremely difficult to inject, due to aggregation, which could not be prevented by the addition of carboxymethylcellulose. This study suggests that larger microspheres are less myotoxic than smaller microspheres.
- Subjects :
- Male
Polymers
medicine.medical_treatment
Myotoxin
Pharmaceutical Science
Biocompatible Materials
Microsphere
Rats, Sprague-Dawley
chemistry.chemical_compound
Muscular Diseases
Polylactic Acid-Polyglycolic Acid Copolymer
medicine
Animals
Lactic Acid
Particle Size
Muscle, Skeletal
Saline
Creatine Kinase
Poly lactide co glycolide
Chromatography
Chemistry
Skeletal muscle
General Medicine
Anatomy
Microspheres
Rats
Solvent
Molecular Weight
PLGA
medicine.anatomical_structure
Distilled water
Polyglycolic Acid
Subjects
Details
- ISSN :
- 10837450
- Volume :
- 1
- Issue :
- 3
- Database :
- OpenAIRE
- Journal :
- Pharmaceutical development and technology
- Accession number :
- edsair.doi.dedup.....99a003b6492da48cdd9b3a0ec3636893