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Ex Vivo Expanded Human NK Cells Survive and Proliferate in Humanized Mice with Autologous Human Immune Cells
- Source :
- Scientific Reports, Vol 7, Iss 1, Pp 1-6 (2017), Scientific Reports
- Publication Year :
- 2017
- Publisher :
- Nature Publishing Group, 2017.
-
Abstract
- Adoptive immune cell therapy is emerging as a promising immunotherapy for cancer. Particularly, the adoptive transfer of NK cells has garnered attention due to their natural cytotoxicity against tumor cells and safety upon adoptive transfer to patients. Although strategies exist to efficiently generate large quantities of expanded NK cells ex vivo, it remains unknown whether these expanded NK cells can persist and/or proliferate in vivo in the absence of exogenous human cytokines. Here, we have examined the adoptive transfer of ex vivo expanded human cord blood-derived NK cells into humanized mice reconstituted with autologous human cord blood immune cells. We report that ex vivo expanded NK cells are able to survive and possibly proliferate in vivo in humanized mice without exogenous cytokine administration, but not in control mice that lack human immune cells. These findings demonstrate that the presence of autologous human immune cells supports the in vivo survival of ex vivo expanded human NK cells. These results support the application of ex vivo expanded NK cells in cancer immunotherapy and provide a translational humanized mouse model to test the lifespan, safety, and functionality of adoptively transferred cells in the presence of autologous human immune cells prior to clinical use.
- Subjects :
- 0301 basic medicine
Cytotoxicity, Immunologic
Adoptive cell transfer
Cell Survival
medicine.medical_treatment
lcsh:Medicine
Biology
Immunotherapy, Adoptive
Article
Cell therapy
03 medical and health sciences
0302 clinical medicine
Immune system
Cancer immunotherapy
Mice, Inbred NOD
Cell Line, Tumor
Neoplasms
medicine
Animals
Humans
lcsh:Science
Cells, Cultured
Cell Proliferation
Mice, Knockout
Multidisciplinary
lcsh:R
Immunotherapy
Fetal Blood
Adoptive Transfer
Killer Cells, Natural
030104 developmental biology
Cytokine
030220 oncology & carcinogenesis
Immunology
Humanized mouse
lcsh:Q
K562 Cells
Ex vivo
Subjects
Details
- Language :
- English
- ISSN :
- 20452322
- Volume :
- 7
- Issue :
- 1
- Database :
- OpenAIRE
- Journal :
- Scientific Reports
- Accession number :
- edsair.doi.dedup.....9995c4d05eef9a55235bb312c79190da