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Effects of a hexokinase II deletion on the dynamics of glycolysis in continuous cultures ofSaccharomyces cerevisiae
- Source :
- FEMS Yeast Research, 2, 165-172. Oxford University Press
- Publication Year :
- 2002
- Publisher :
- Oxford University Press (OUP), 2002.
-
Abstract
- In glucose-limited aerobic chemostat cultures of a wild-type Saccharomyces cerevisiae and a derived hxk2 null strain, metabolic fluxes were identical. However, the concentrations of intracellular metabolites, especially fructose 1,6-bisphosphate, and hexose-phosphorylating activities differed. Interestingly, the hxk2 null strain showed a higher maximal growth rate and higher Crabtree threshold dilution rate, revealing a higher oxidative capacity for this strain. After a pulse of glucose, aerobic glucose-limited cultures of wild-type S. cerevisiae displayed an overshoot in the intracellular concentrations of glucose 6-phosphate, fructose 6-phosphate, and fructose 1,6-bisphosphate before a new steady state was established, in contrast to the hxk2 null strain which reached a new steady state without overshoot of these metabolites. At low dilution rates the overshoot of intracellular metabolites in the wild-type strain coincided with the immediate production of ethanol after the glucose pulse. In contrast, in the hxk2 null strain the production of ethanol started gradually. However, in spite of the initial differences in ethanol production and dynamic behaviour of the intracellular metabolites, the steady-state fluxes after transition from glucose limitation to glucose excess were not significantly different in the wild-type strain and the hxk2 null strain at any dilution rate.
- Subjects :
- Hexokinase
Strain (chemistry)
Genes, Fungal
Fructose
Saccharomyces cerevisiae
General Medicine
Chemostat
Biology
Applied Microbiology and Biotechnology
Microbiology
Aerobiosis
Culture Media
chemistry.chemical_compound
Glucose
Biochemistry
chemistry
Glucose 6-phosphate
Crabtree effect
Glycolysis
Steady state (chemistry)
Gene Deletion
Subjects
Details
- ISSN :
- 15671364 and 15671356
- Volume :
- 2
- Database :
- OpenAIRE
- Journal :
- FEMS Yeast Research
- Accession number :
- edsair.doi.dedup.....99818f01316fbf519600dbeb585e61dc