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5-azacytidine improves the osteogenic differentiation potential of aged human adipose-derived mesenchymal stem cells by DNA demethylation
- Source :
- Yan, X, Ehnert, S, Culmes, M, Bachmann, A, Seeliger, C, Schyschka, L, Wang, Z, Rahmanian-Schwarz, A, Stöckle, U, De Sousa, P A, Pelisek, J & Nussler, A K 2014, ' 5-azacytidine improves the osteogenic differentiation potential of aged human adipose-derived mesenchymal stem cells by DNA demethylation ', PLoS ONE, vol. 9, no. 3, pp. e90846 . https://doi.org/10.1371/journal.pone.0090846, PLoS ONE, Vol 9, Iss 6, p e90846 (2014), PLoS ONE
- Publication Year :
- 2014
-
Abstract
- The therapeutic value of adipose-derived mesenchymal stem cells (Ad-MSCs) for bone regeneration is critically discussed. A possible reason for reduced osteogenic potential may be an age-related deterioration of the Ad-MSCs. In long term in vitro culture, epigenomic changes in DNA methylation are known to cause gene silencing, affecting stem cell growth as well as the differentiation potential. In this study, we observed an age-related decline in proliferation of primary human Ad-MSCs. Decreased Nanog, Oct4 and Lin28A and increased Sox2 gene-expression was accompanied by an impaired osteogenic differentiation potential of Ad-MSCs isolated from old donors (>60 a) as compared to Ad-MSCs isolated from younger donors (
- Subjects :
- Aging
Anatomy and Physiology
Cellular differentiation
medicine.medical_treatment
Gene Expression
lcsh:Medicine
Core Binding Factor Alpha 1 Subunit
Epigenesis, Genetic
Osteogenesis
Molecular Cell Biology
lcsh:Science
DNA Modification Methylases
Cells, Cultured
Multidisciplinary
Stem Cells
Cell Differentiation
Stem-cell therapy
DNA-Binding Proteins
Sp7 Transcription Factor
DNA methylation
Azacitidine
Medicine
Epigenetics
Stem cell
Cellular Types
DNA modification
Research Article
Homeobox protein NANOG
Adipose Tissue, White
Osteocalcin
Biology
Dioxygenases
SOX2
Proto-Oncogene Proteins
medicine
Genetics
Humans
Cell Proliferation
Mesenchymal stem cell
lcsh:R
Mesenchymal Stem Cells
DNA Methylation
Molecular biology
DNA demethylation
Cancer research
lcsh:Q
Physiological Processes
Transcription Factors
Developmental Biology
Subjects
Details
- Language :
- English
- Database :
- OpenAIRE
- Journal :
- Yan, X, Ehnert, S, Culmes, M, Bachmann, A, Seeliger, C, Schyschka, L, Wang, Z, Rahmanian-Schwarz, A, Stöckle, U, De Sousa, P A, Pelisek, J & Nussler, A K 2014, ' 5-azacytidine improves the osteogenic differentiation potential of aged human adipose-derived mesenchymal stem cells by DNA demethylation ', PLoS ONE, vol. 9, no. 3, pp. e90846 . https://doi.org/10.1371/journal.pone.0090846, PLoS ONE, Vol 9, Iss 6, p e90846 (2014), PLoS ONE
- Accession number :
- edsair.doi.dedup.....9968d2723a3b7c82f451ef58468d1070