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The differentially DNA-methylated region responsible for expression of runt-related transcription factor 2
- Source :
- The Journal of Veterinary Medical Science
- Publication Year :
- 2017
- Publisher :
- Japanese Society of Veterinary Science, 2017.
-
Abstract
- Runt-related transcription factor 2 (Runx2) is essential for osteogenesis. This study aimes at identification of the genomic region differentially methylated in DNA for regulation of Runx2 expression. In the proximal promoter of mouse Runx2, DNA methylation was frequent at the region further than 3 kb relative to the transcription start site, in contrast to lower methylation status of the closer locus within 2 kb from the transcription start site. At the intermediate part, we identified a novel differentially methylated region in the Runx2 promoter region (Runx2-DMR): from -2.7 to -2.2 kb relative to the start site of Runx2 transcription in mice. In this region, the DNA methylation rate correlated negatively with Runx2 expression among mouse organs as well as among primary cultures of bone marrow from different dogs. Induction of mouse and dog mesenchymal-like cells into osteoblastic differentiation decreased the methylation rate of Runx2-DMR. Thus, in this study, we identified a novel genomic region in which DNA methylation status is related to Runx2 expression and detected demethylation of Runx2-DMR during osteoblastic differentiation in mouse and dog.
- Subjects :
- Male
musculoskeletal diseases
0301 basic medicine
correlation analysis
Response element
Bone Marrow Cells
Core Binding Factor Alpha 1 Subunit
Biology
osteogenesis
03 medical and health sciences
Dogs
Epigenetics of physical exercise
Species Specificity
stomatognathic system
Runx2
Transcription (biology)
Animals
Promoter Regions, Genetic
Transcription factor
Cells, Cultured
DNA methylation
promoter
Osteoblasts
Full Paper
General Veterinary
musculoskeletal, neural, and ocular physiology
Cell Differentiation
Promoter
Methylation
musculoskeletal system
Molecular biology
Mice, Inbred C57BL
030104 developmental biology
Differentially methylated regions
Gene Expression Regulation
embryonic structures
Anatomy
Subjects
Details
- ISSN :
- 13477439 and 09167250
- Volume :
- 79
- Database :
- OpenAIRE
- Journal :
- Journal of Veterinary Medical Science
- Accession number :
- edsair.doi.dedup.....9955e2b11d79ce6c69fb546f0fc1e48d